Surface defects due to bacterial residue on shrimp shell.

Autor: Celebi Ö; Department of Microbiology, Faculty of Veterinary Medicine Kafkas University, 36000 Kars, Turkey., Bahadir T; Department of Environmental Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey., Şimşek İ; Department of Environmental Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey., Aydın F; Department of Food Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey., Kahve Hİ; Department of Food Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey., Tulun Ş; Department of Environmental Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey., Büyük F; Department of Microbiology, Faculty of Veterinary Medicine Kafkas University, 36000 Kars, Turkey., Celebi H; Department of Environmental Engineering, Faculty of Engineering, Aksaray University, 68100 Aksaray, Turkey. Electronic address: hakanaz.celebi@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Apr; Vol. 263 (Pt 2), pp. 130353. Date of Electronic Publication: 2024 Feb 24.
DOI: 10.1016/j.ijbiomac.2024.130353
Abstrakt: The changes in the surface chemistry and morphological structure of chitin forms obtained from shrimp shells (ShpS) with and without microorganisms were evaluated. Total mesophilic aerobic bacteria (TMAB), estimated Pseudomonas spp. and Enterococcus spp. were counted in Shp-S by classical cultural counting on agar medium, where the counts were 6.56 ± 0.09, 6.30 ± 0.12, and 3.15 ± 0.03 CFU/g, respectively. Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM)/Energy dispersed X-ray (EDX) were used to assess the surface chemistry/functional groups and morphological structure for ChT free (non-microorganism), and ChT mo (with microorganisms). ChT free FTIR spectra presented a detailed chitin structure by OH, NH, and CO stretching vibrations, whereas specific peaks of chitin could not be detected in ChT mo . Major differences were also found in SEM analysis for ChT free and ChT mo . ChT free had a flat, prominent micropore, partially homogeneous structure, while ChT mo had a layered, heterogeneous, complex dense fibrous, and lost pores form. The degree of deacetylation was calculated for ChT free and ChT mo according to FTIR and EDX data. The results suggest that the degree of deacetylation decreases in the presence of microorganisms, affecting the production of beneficial components negatively. The findings were also supported by the molecular docking model.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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