Bacterial defense systems exhibit synergistic anti-phage activity.

Autor:	Wu Y; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK., Garushyants SK; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., van den Hurk A; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK., Aparicio-Maldonado C; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK., Kushwaha SK; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK; Department of Biological Sciences, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan, India., King CM; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK., Ou Y; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK., Todeschini TC; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK., Clokie MRJ; Department of Genetics and Genome Biology, University of Leicester, Leicester, UK., Millard AD; Department of Genetics and Genome Biology, University of Leicester, Leicester, UK., Gençay YE; SNIPR biome, Copenhagen, Denmark., Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Nobrega FL; School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, UK. Electronic address: f.nobrega@soton.ac.uk.
Jazyk:	angličtina
Zdroj:	Cell host & microbe [Cell Host Microbe] 2024 Apr 10; Vol. 32 (4), pp. 557-572.e6. Date of Electronic Publication: 2024 Feb 22.
DOI:	10.1016/j.chom.2024.01.015
Abstrakt:	Bacterial defense against phage predation involves diverse defense systems acting individually and concurrently, yet their interactions remain poorly understood. We investigated >100 defense systems in 42,925 bacterial genomes and identified numerous instances of their non-random co-occurrence and negative association. For several pairs of defense systems significantly co-occurring in Escherichia coli strains, we demonstrate synergistic anti-phage activity. Notably, Zorya II synergizes with Druantia III and ietAS defense systems, while tmn exhibits synergy with co-occurring systems Gabija, Septu I, and PrrC. For Gabija, tmn co-opts the sensory switch ATPase domain, enhancing anti-phage activity. Some defense system pairs that are negatively associated in E. coli show synergy and significantly co-occur in other taxa, demonstrating that bacterial immune repertoires are largely shaped by selection for resistance against host-specific phages rather than negative epistasis. Collectively, these findings demonstrate compatibility and synergy between defense systems, allowing bacteria to adopt flexible strategies for phage defense. Competing Interests: Declaration of interests Y.E.G. is a full-time employee of SNIPR Biome. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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