Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock.

Autor: Keita VM; Department of Chemical & Biological Engineering, University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield, S1 3JD, UK.; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore, 138668, Singapore., Lee YQ; School of Chemical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea., Lakshmanan M; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore, 138668, Singapore.; Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, 600036, India., Ow DS; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Centros, Singapore, 138668, Singapore., Staniland P; Croda Europe Ltd., Oak Road, Clough Road, Hull, HU6 7PH, UK., Staniland J; Croda Europe Ltd., Oak Road, Clough Road, Hull, HU6 7PH, UK., Savill I; Croda Europe Ltd., Oak Road, Clough Road, Hull, HU6 7PH, UK., Tee KL; Department of Chemical & Biological Engineering, University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield, S1 3JD, UK., Wong TS; Department of Chemical & Biological Engineering, University of Sheffield, Sir Robert Hadfield Building, Mappin Street, Sheffield, S1 3JD, UK. t.wong@sheffield.ac.uk.; Evolutor Ltd, The Innovation Centre, 217 Portobello, Sheffield, S1 4DP, UK. t.wong@sheffield.ac.uk.; National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Phahonyothin Road, Khlong Nueang, Khlong Luang, 12120, Pathum Thani, Thailand. t.wong@sheffield.ac.uk.; School of Pharmacy, Bandung Institute of Technology, 10 Coblong, Bandung, West Java, 40132, Indonesia. t.wong@sheffield.ac.uk., Lee DY; School of Chemical Engineering, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea. dongyuplee@skku.edu.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2024 Feb 24; Vol. 23 (1), pp. 63. Date of Electronic Publication: 2024 Feb 24.
DOI: 10.1186/s12934-024-02336-x
Abstrakt: Background: Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH.
Results: We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation.
Conclusion: Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production.
(© 2024. The Author(s).)
Databáze: MEDLINE
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