Isotachophoretic quantification of total viable bacteria on meat and surfaces.

Autor: Kumarajith TM; Australia Centre for Research on Separation Science, Chemistry, School of Natural Sciences, Tasmania, Australia; Tasmanian Institute of Agriculture, Tasmania, Australia., Powell SM; Tasmanian Institute of Agriculture, Tasmania, Australia., Breadmore MC; Australia Centre for Research on Separation Science, Chemistry, School of Natural Sciences, Tasmania, Australia. Electronic address: michael.breadmore@utas.edu.au.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Apr 01; Vol. 1296, pp. 342253. Date of Electronic Publication: 2024 Jan 20.
DOI: 10.1016/j.aca.2024.342253
Abstrakt: Background: The quantification of microbes, particularly live bacteria, is of utmost importance in assessing the quality of meat products. In the context of meat processing facilities, prompt identification and removal of contaminated carcasses or surfaces is crucial to ensuring the continuous production of safe meat for human consumption. The plate count method and other traditional detection methods are not only labour-intensive but also time-consuming taking 24-48 h.
Results: In this report, we present a novel isotachophoretic quantification method utilizing two nucleic acid stains, SYTO9 and propionic iodide, for the detection of total viable bacteria. The study employed E. coli M23 bacteria as a model organism, with an analysis time of only 30 min. The method demonstrated a limit of detection (LOD) of 184 CFU mL -1 and 14 cells mL -1 for total viable count and total cell count, respectively. Furthermore, this new approach is capable of detecting the microbial quality standard limits for food contacting surfaces (10 CFU cm -2 ) and meat (1.99 × 10 4  CFU cm -2 ) by swabbing an area of 10 × 10 cm 2 .
Significance: In contrast to the culture-based methods usually employed in food processing facilities, this isotachophoretic technique enables easy and rapid detection (<30 min) of microorganisms, facilitating crucial decision-making essential for maintaining product quality and safety.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Michael Breadmore reports financial support was provided by Australian Meat Processor Corporation. Shane Powell reports financial support was provided by Australian Meat Processor Corporation. Michael Breadmore serves as an editor on ACA. Thisara Kumarajithdeclares no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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