Induction of human-fetal-membrane remodeling in-vitro by the alpha hemolysin of Escherichia coli.

Autor: Pucci Molineris M; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Av. 60 &120, La Plata, Buenos Aires, Argentina. Electronic address: mpucci@med.unlp.edu.ar., Schibert F; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Av. 60 &120, La Plata, Buenos Aires, Argentina., Lima M; Cátedra B de Patología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Av. 60 &120, La Plata, Buenos Aires, Argentina., Accialini P; Laboratorio de Fisiopatología Placentaria, CEFyBO -Facultad de Medicina, Universidad de Buenos Aires, Paraguay N° 2155, Ciudad Autónoma de Buenos Aires, Argentina., Cané L; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Av. 60 &120, La Plata, Buenos Aires, Argentina., Pelinsky P; Servicio de Ginecología y Obstetricia, Hospital Español de La Plata, 9 N° 175, La Plata, Buenos Aires, Argentina., Farina M; Laboratorio de Fisiopatología Placentaria, CEFyBO -Facultad de Medicina, Universidad de Buenos Aires, Paraguay N° 2155, Ciudad Autónoma de Buenos Aires, Argentina., Herlax V; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Av. 60 &120, La Plata, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Placenta [Placenta] 2024 Mar 25; Vol. 148, pp. 59-68. Date of Electronic Publication: 2024 Feb 13.
DOI: 10.1016/j.placenta.2024.02.001
Abstrakt: Introduction: Almost 80% of urinary tract infections during pregnancy are caused by uropathogenic strains of Escherichia coli. Alpha-hemolysin, toxin secreted by them, has a fundamental role in this pathology development. Considering that urinary tract infections are related with premature rupture of fetal membranes, we proposed to evaluate the effects that alpha-hemolysin induces on human-fetal-membranes.
Methods: Thirteen fetal membranes obtained from elective cesarean sections (>37 weeks) were mounted in a transwell-device generating two independent chambers. To mimic an ascendant-urinary-tract infection, membranes were incubated with different concentrations of pure alpha-hemolysin from the choriodecidual side during 24h. Extensive histological analyses were performed and transepithelial electrical-resistance were determined. Cell viability, metalloproteinase activity and cyclooxygenase-2- gene expression was estimated by lactate-dehydrogenase-release assay, zymography and RT-qPCR, respectively. Finally, four fetal membranes were treated with hemolysin preincubated with polyclonal anti-hemolysin antibodies. Cell viability and metalloproteinase activity were monitored.
Results: After 24 h of treatment, fetal membranes evidenced a structural damage and a decrease in membrane resistance that progressed as the concentration of alpha hemolysin increased. While the amniotic-epithelial-layer remained practically unaffected, the chorion cells manifested an increase in vacuolization and necrosis. In addition, the extracellular matrix exhibited collagen-fiber disorganization, a marked decrease in fiber content, and became thicker in presence of the toxin. Cyclooxigenase-2 expression and metalloproteinase activity were also higher in the treated groups than in untreated ones. Finally, a preincubation of hemolysin with specific antibodies prevented the cytotoxicity on the chorion cells and the increase in metalloproteinase activity.
Discussion: Hemolysin induces structural and molecular changes associated with the remodeling of human-fetal-membranes in-vitro.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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