Exploring miR-155-5p and miR-1246 as Diagnostic and Prognostic Markers in Oral Squamous cell carcinoma.

Autor: Maheswari R; Department of Oral Pathology, Maulana Azad Institute of Dental Sciences, New Delhi, 110002, India., Urs AB; Department of Oral Pathology, Maulana Azad Institute of Dental Sciences, New Delhi, 110002, India., Kumar P; Department of Oral Pathology, Maulana Azad Institute of Dental Sciences, MAMC Complex, Mirdard Marg, Bahadur Shah Zafar Marg, LNJP Colony, New Delhi, 110002, India. drpri_kumar@yahoo.com., Koner BC; Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India., Guru SA; Developmental Systems Biology, Ann and Lurie Children's Hospital, Northwestern University, Chicago, IL, 60611, USA., Rawat G; Department of Pathology, Dharamshila Narayana Superspeciality Hospital, New Delhi, 110002, India.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Feb 24; Vol. 51 (1), pp. 341. Date of Electronic Publication: 2024 Feb 24.
DOI: 10.1007/s11033-024-09234-w
Abstrakt: Introduction: Oral Squamous Cell Carcinoma (OSCC) is one of the leading cancers worldwide, significantly impacting developing nations. This study aimed to explore the diagnostic and prognostic potential of miR-155-5p and miR-1246 in OSCC in the Indian population, as their comparative roles in this context remain unexplored.
Material and Methods: The present cross-sectional study comprised 50 histopathologically confirmed OSCC cases, with adjacent normal mucosa as controls. MiRNA expression was assessed via qRT-PCR and correlated with clinicopathological factors. MiRwalk and miRTargetlink were used for miRNA:mRNA interaction prediction, and gprofiler was employed to analyze validated targets for functional insights.
Results: The expression analysis showed a significant upregulation of miR-155-5p and miR-1246 in OSCC tissues compared to adjacent controls. Receiver operating curve analysis revealed that miR-1246 exhibited excellent diagnostic accuracy (AUC = 0.94) compared to miR-155-5p (AUC = 0.69). Higher miRNA levels were associated with age and extracapsular extension while overexpression of miR-1246 was correlated significantly with increased tumor size, tumor grade, TNM staging, and depth of invasion. The analysis for target prediction unveiled a set of validated targets, among which were WNT5A, TP53INP1, STAT3, CTNNB1, PRKAR1A, and NFIB.
Conclusion: miR-155-5p and miR-1246 may be used as potential prognostic biomarkers in OSCC, with miR-1246 demonstrating superior diagnostic accuracy.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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