| Autor: |
Kaczmarek-Szczepańska B; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland., Zasada L; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland., Wekwejt M; Department of Biomaterials Technology, Faculty of Mechanical Engineering and Ship Technology, Gdańsk University of Technology, 80-233 Gdańsk, Poland., Brzezinska MS; Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87-100 Torun, Poland., Michno A; Department of Molecular Medicine, Medical University of Gdańsk, 80-210 Gdańsk, Poland., Ronowska A; Department of Molecular Medicine, Medical University of Gdańsk, 80-210 Gdańsk, Poland., Ciesielska M; Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University, Gagarin 7, 87-100 Toruń, Poland., Kovtun G; Institute of Magnetism NAS of Ukraine and MES of Ukraine, Blvd. Acad. Vernadsky 36-b, 03142 Kyiv, Ukraine.; Department of Applied Mechanics and Project Engineering, Mining and Industrial Engineering School of Almaden, University of Castilla-La Mancha, Plaza Manuel Meca 1, 13400 Almadén, Spain., Cuberes MT; Department of Applied Mechanics and Project Engineering, Mining and Industrial Engineering School of Almaden, University of Castilla-La Mancha, Plaza Manuel Meca 1, 13400 Almadén, Spain. |
| Abstrakt: |
Bioactive materials may be applied in tissue regeneration, and an example of such materials are wound dressings, which are used to accelerate skin healing, especially after trauma. Here, we proposed a novel dressing enriched by a bioactive component. The aim of our study was to prepare and characterize poly(vinyl alcohol) films modified with strontium titanate nanoparticles. The physicochemical properties of films were studied, such as surface free energy and surface roughness, as well as the mechanical properties of materials. Moreover, different biological studies were carried out, like in vitro hemo- and cyto-compatibility, biocidal activity, and anti-biofilm formation. Also, the degradation of the materials' utilization possibilities and enzymatic activity in compost were checked. The decrease of surface free energy, increase of roughness, and improvement of mechanical strength were found after the addition of nanoparticles. All developed films were cyto-compatible, and did not induce a hemolytic effect on the human erythrocytes. The PVA films containing the highest concentration of STO (20%) reduced the proliferation of Eschericha coli , Pseudomonas aeruginosa , and Staphylococcus aureus significantly. Also, all films were characterized by surface anti-biofilm activity, as they significantly lowered the bacterial biofilm abundance and its dehydrogenase activity. The films were degraded by the compost microorganism. However, PVA with the addition of 20%STO was more difficult to degrade. Based on our results, for wound dressing application, we suggest using bioactive films based on PVA + 20%STO, as they were characterized by high antibacterial properties, favorable physicochemical characteristics, and good biocompatibility with human cells. |
