The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against Candida albicans : An Ex Vivo Analysis.

Autor: Alghofaily M; Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia., Alfraih J; College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia., Alsaud A; College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia., Almazrua N; College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia., Sumague TS; Molecular and Cell Biology Laboratory, Prince Naif Bin AbdulAziz Health Research Center, College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia., Auda SH; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11461, Saudi Arabia., Alsalleeh F; Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11461, Saudi Arabia.
Jazyk: angličtina
Zdroj: Microorganisms [Microorganisms] 2024 Jan 29; Vol. 12 (2). Date of Electronic Publication: 2024 Jan 29.
DOI: 10.3390/microorganisms12020289
Abstrakt: Introduction: The purpose of this study was to assess the antifungal activity of silver nanoparticles (AgNPs) in combination with calcium hydroxide (Ca(OH) 2 ) against Candida albicans ( C. albicans ).
Methods: AgNPs was mixed with pure Ca(OH) 2 powder in an aqueous base. A standard suspension (1 × 10 8 bacterial cells/mL) of C. albicans was prepared in a 96-well plate and incubated on shaker at 37 °C in 100% humidity to allow fungal biofilm formation in infected dentin slices ( n = 98). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of AgNPs alone or with Ca(OH) 2 were determined. The samples were separately placed in 24-well tissue culture plates and divided into three experimental groups (0.03, 0.04, and 0.06) and three control groups; negative (saline) and positive chlorhexidine gel and Ca(OH) 2 . Quantitative measurements of fungal activity by XTT colorimetric assay and qualitative measurements using confocal laser microscopy and scanning electron microscopy were performed.
Results: The cell viability of C. albicans in the experimental groups was significantly reduced compared to the negative control group. The combination of (AgNPs (0.04%) and Ca(OH) 2 ) was the most potent against C. albicans .
Conclusions: The findings demonstrated that combining silver nanoparticles with Ca(OH) 2 was more effective against C. albicans biofilm compared to Ca(OH) 2 alone, suggesting a combing effect.
Databáze: MEDLINE
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