| Autor: |
Gaber Y; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Al-Karak 61710, Jordan.; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt., TumAllah HM; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Mutah University, Al-Karak 61710, Jordan., AbdelAllah NH; Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt.; Clinical Trials Department, Central Administrative of Biological, Innovative Products and Clinical Trials, Egyptian Drug Authority, Giza 12654, Egypt., Al-Zereini WA; Department of Biological Sciences, Faculty of Sciences, Mutah University, Al-Karak 61710, Jordan., Abu-Lubad MA; Department of Microbiology and Pathology, Faculty of Medicine, Mutah University, Al-Karak 61710, Jordan., Aqel AA; Department of Microbiology and Pathology, Faculty of Medicine, Mutah University, Al-Karak 61710, Jordan., Elkhatib WF; Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, African Union Organization St., Abbassia, Cairo 11566, Egypt.; Department of Microbiology & Immunology, Faculty of Pharmacy, Galala University, New Galala City, Suez 43713, Egypt., Goering RV; Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178, USA., Soliman AM; Department of Microbiology and Immunology, Faculty of Pharmacy, Kafr-Elsheikh University, Kafr El-Sheikh 33516, Egypt. |
| Abstrakt: |
Background and Objectives : Methicillin-resistant Staphylococcus aureus (MRSA) is a major concern in Jordanian hospitals in terms of infection control. The purpose of this study was to identify the resistance patterns of Staphylococcus aureus strains isolated from surfaces of critical locations within the Al-Karak Governmental Hospital in 2019. Additionally, the study aimed to conduct whole-genome sequencing on the isolates. Materials and Methods : In February 2019, fourteen S. aureus strains were isolated from surfaces in critical sites in the Al-Karak Governmental Hospital. These isolates underwent antibiogram testing to determine their resistance profile. Genome sequencing using the Illumina MiSeq platform was applied to the extracted DNA from these isolates. The genomic data, including coding sequences, were analyzed to identify lineage, resistance genes, and plasmids. Results : The antibiogram results revealed that 11 of the 14 isolates were resistant to oxacillin, 6 to linezolid, and 1 to rifampicin, while none showed resistance to chloramphenicol. Eleven isolates were identified as MRSA, with a novel spa type (t4407) not previously reported in Jordan. High-quality sequencing data were obtained for only one isolate, i.e., A29, the genome showed 2,789,641 bp with a 32.7% GC content and contained 2650 coding sequences. Genomic analysis indicated the ST6 lineage, mecA gene (SCC mec type IVa(2B)), and a hybrid plasmid (pJOR_ blaZ ) carrying the blaZ gene for β-lactam resistance. Genomic data were deposited in NCBI (CP104989). The A29 genome closely resembled an MRSA genome isolated from a Danish hospital in 2011. The SNP analysis revealed identical antimicrobial resistance genes in these two genomes. Conclusions : This study unveils the first genomic sequence of an MRSA isolate from Jordan, marked by distinctive genotypic traits. The findings enhance our understanding of the MRSA types circulating in Jordan and the region and substantiate the phenomenon of intercontinental MRSA transmission. |
