Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues.

Autor: Krivoruchko AA; Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, Russia., Zdorovenko EL; N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Ivanova MF; Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, Russia., Kostina EE; Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, Russia., Fedonenko YP; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia.; Department of Biochemistry and Biophysics, Faculty of Biology, Saratov State University, 410012 Saratov, Russia., Shashkov AS; N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Dmitrenok AS; N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Ul'chenko EA; Department of Biomedical Products, Faculty of Chemical Pharmaceutical Technologies, D.I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia., Tkachenko OV; Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, Russia., Astankova AS; Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, Russia., Burygin GL; Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, Russia.; Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, Russia.; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, Russia.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Feb 06; Vol. 25 (4). Date of Electronic Publication: 2024 Feb 06.
DOI: 10.3390/ijms25041970
Abstrakt: Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of Ochrobactrum quorumnocens T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical-chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-d-Fuc f -(1→3)-β-d-Fuc p -(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-d-Glc p -(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of d-fucofuranose residues at position 3 were O-acetylated, and 15% of d-Glc p residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both d-fucopyranose and d-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of -21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of O. quorumnocens T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of d-fucose.
Databáze: MEDLINE
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