| Autor: |
Duarte da Silva KC; Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., Carneiro WF; Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., Virote BDCR; Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., Santos MF; School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., de Oliveira JPL; School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., Castro TFD; Institute of Biomedical Sciences II (ICBII), Universidade de São Paulo, São Paulo 05508-000, São Paulo, Brazil., Bertolucci SKV; School of Agricultural Sciences of Lavras (ESAL), Department of Agriculture, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil., Murgas LDS; Faculty of Animal Science and Veterinary Medicine (FZMV), Department of Veterinary Medicine, Federal University of Lavras, Lavras 37200-900, Minas Gerais, Brazil. |
| Abstrakt: |
This study explored the protective capacity of the essential oil (EO) of Cymbopogon citratus against oxidative stress induced by hydrogen peroxide (H 2 O 2 ) and the inflammatory potential in zebrafish. Using five concentrations of EO (0.39, 0.78, 1.56, 3.12, and 6.25 μg/mL) in the presence of 7.5 mM H 2 O 2 , we analyzed the effects on neutrophil migration, caudal fin regeneration, cellular apoptosis, production of reactive oxygen species (ROS), and activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) after 96 h of exposure. A significant decrease in neutrophil migration was observed in all EO treatments compared to the control. Higher concentrations of EO (3.12 and 6.25 μg/mL) resulted in a significant decrease in caudal fin regeneration compared to the control. SOD activity was reduced at all EO concentrations, CAT activity significantly decreased at 3.12 μg/mL, and GST activity increased at 0.78 μg/mL and 1.56 μg/mL, compared to the control group. No significant changes in ROS production were detected. A reduction in cellular apoptosis was evident at all EO concentrations, suggesting that C . citratus EO exhibits anti-inflammatory properties, influences regenerative processes, and protects against oxidative stress and apoptosis. |
