Metabarcoding study to reveal the structural community of strongylid nematodes in domesticated horses in Thailand.

Autor: Hamad MH; The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.; Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.; Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand., Islam SI; The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.; Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand., Jitsamai W; Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok, Thailand., Chinkangsadarn T; Department of Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand., Naraporn D; Horse Farm and Laboratory Animal Breeding Center, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Hua-Hin, Prachuap Khiri Khan Province, 77110, Thailand., Ouisuwan S; Horse Farm and Laboratory Animal Breeding Center, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Hua-Hin, Prachuap Khiri Khan Province, 77110, Thailand., Taweethavonsawat P; Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand. Piyanan.T@chula.ac.th.; Biomarkers in Animals Parasitology Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand. Piyanan.T@chula.ac.th.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Feb 24; Vol. 20 (1), pp. 70. Date of Electronic Publication: 2024 Feb 24.
DOI: 10.1186/s12917-024-03934-y
Abstrakt: Background: Mixed strongylid infections significantly impact equine health and performance. Traditional microscopy-based methods exhibit limitations in accurately identifying strongylid species. Nemabiome deep amplicon sequencing approach previously succeeded in describing the strongylid communities in livestock including equids. However, there are no available studies that describe the structural communities of strongylid parasites in horses in Thailand. Therefore, this study was undertaken encompassing the ITS-2 rDNA metabarcoding assay to characterize strongylid species within horse fecal samples collected from a cohort of yearlings at the largest domesticated stud farm in Thailand. In addition, to investigate the capability of ITS-2 rDNA in assessing the phylogenetic relationships among the identified strongylid species.
Results: The study identified 14 strongylid species in the examined equine populations, each with varying prevalence. Notably, Cylicocyclus nassatus and Cylicostephanus longibursatus were identified as the predominant species, with Strongylus spp. conspicuously absent. The phylogenetic analysis of 207 amplicon sequence variants (ASVs) displayed a complex relationship among the investigated cyathostomin species, with some species are positioned across multiple clades, demonstrating close associations with various species and genera.
Conclusion: The ITS-2 nemabiome sequencing technique provided a detailed picture of horse strongylid parasite species in the studied population. This establishes a foundation for future investigations into the resistance status of these parasites and enables efforts to mitigate their impact.
(© 2024. The Author(s).)
Databáze: MEDLINE
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