Structure of a unique PSII-Pcb tetrameric megacomplex in a chlorophyll d -containing cyanobacterium.

Autor: Shen L; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China.; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.; China National Botanical Garden, Beijing 100093, China., Gao Y; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Tang K; Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China., Qi R; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Fu L; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Chen JH; Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.; College of Life Sciences, Zhejiang University, Hangzhou 310058, China., Wang W; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.; China National Botanical Garden, Beijing 100093, China., Ma X; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Li P; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Chen M; School of Life and Environmental Science, Faculty of Science, University of Sydney, Sydney NSW 2006, Australia., Kuang T; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.; China National Botanical Garden, Beijing 100093, China., Zhang X; Department of Biophysics, and Department of Pathology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.; Center of Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou 310058, China.; Zhejiang Laboratory for System and Precision Medicine, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou 311121, China., Shen JR; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.; China National Botanical Garden, Beijing 100093, China.; Institute for Interdisciplinary Science, and Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan., Wang P; Cryo-EM Centre, Southern University of Science and Technology, Shenzhen 518055, China., Han G; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.; China National Botanical Garden, Beijing 100093, China.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2024 Feb 23; Vol. 10 (8), pp. eadk7140. Date of Electronic Publication: 2024 Feb 23.
DOI: 10.1126/sciadv.adk7140
Abstrakt: Acaryochloris marina is a unique cyanobacterium using chlorophyll d (Chl d ) as its major pigment and thus can use far-red light for photosynthesis. Photosystem II (PSII) of A. marina associates with a number of prochlorophyte Chl-binding (Pcb) proteins to act as the light-harvesting system. We report here the cryo-electron microscopic structure of a PSII-Pcb megacomplex from A. marina at a 3.6-angstrom overall resolution and a 3.3-angstrom local resolution. The megacomplex is organized as a tetramer consisting of two PSII core dimers flanked by sixteen symmetrically related Pcb proteins, with a total molecular weight of 1.9 megadaltons. The structure reveals the detailed organization of PSII core consisting of 15 known protein subunits and an unknown subunit, the assembly of 4 Pcb antennas within each PSII monomer, and possible pathways of energy transfer within the megacomplex, providing deep insights into energy transfer and dissipation mechanisms within the PSII-Pcb megacomplex involved in far-red light utilization.
Databáze: MEDLINE