Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis.

Autor:	El Jailani S; Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France., Wassmann K; Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France., Touati SA; Université Paris Cité, CNRS, Institut Jacques Monod, Paris, France. sandra.touati@ijm.fr.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2740, pp. 211-227.
DOI:	10.1007/978-1-0716-3557-5_13
Abstrakt:	Whole-mount immunofluorescence allows direct visualization of the cellular architecture within cells. Here, we apply this technique to mouse oocytes to visualize spindle morphology and microtubule attachments to kinetochores, using a technique we call "cold treatment," at various phases of the meiotic cell cycle. This method allows the analysis of spindle structures at different meiosis I stages and at metaphase II. An adaptation of the protocol to the cell cycle stage of interest is described. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu