| Autor: |
Olguín JE; Laboratorio Nacional en Salud: Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Estado de México, Mexico., Corano-Arredondo E; Laboratorio Nacional en Salud: Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Estado de México, Mexico., Hernández-Gómez V; Laboratorio Nacional en Salud: Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Estado de México, Mexico., Rivera-Montoya I; Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM, Tlalnepantla 54090, Estado de México, Mexico., Rodríguez MA; Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM, Tlalnepantla 54090, Estado de México, Mexico., Medina-Andrade I; Center for Infectious Medicine (CIM), Department of Medicine, Hudinge, Karolinska Institutet, 141 52 Stockholm, Sweden., Arendse B; Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town 7925, South Africa., Brombacher F; Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town 7925, South Africa., Terrazas LI; Laboratorio Nacional en Salud: Diagnóstico Molecular y Efecto Ambiental en Enfermedades Crónico-Degenerativas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Estado de México, Mexico.; Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, UNAM, Tlalnepantla 54090, Estado de México, Mexico. |
| Abstrakt: |
To determine the role that the IL-4/IL13 receptor plays in the development of alternatively activated macrophages (AAM or M2) and their role in the regulation of immunity to the extraintestinal phase of the helminth parasite Taenia crassiceps , we followed the infection in a mouse strain lacking the IL-4Rα gene (IL-4Rα -/- ) and in the macrophage/neutrophil-specific IL-4Rα-deficient mouse strain (LysMcreIL-4Rα -/lox or cre/LoxP). While 100% of T. crassiceps -infected IL-4Rα +/+ (WT) mice harbored large parasite loads, more than 50% of th eIL-4Rα -/- mice resolved the infection. Approximately 88% of the LysMcreIL-4Rα -/lox mice displayed a sterilizing immunity to the infection. The remaining few infected cre/LoxP mice displayed the lowest number of larvae in their peritoneal cavity. The inability of the WT mice to control the infection was associated with antigen-specific Th2-type responses with higher levels of IgG1, IL-4, IL-13, and total IgE, reduced NO production, and increased arginase activity. In contrast, IL-4Rα -/- semi-resistant mice showed a Th1/Th2 combined response. Furthermore, macrophages from the WT mice displayed higher transcripts for Arginase-1 and RELM-α, as well as increased expression of PD-L2 with robust suppressive activity over anti-CD3/CD28 stimulated T cells; all of these features are associated with the AAM or M2 macrophage phenotype. In contrast, both the IL-4Rα -/- and LysMcreIL-4Rα -/lox mice did not fully develop AAM or display suppressive activity over CD3/CD28 stimulated T cells, reducing PDL2 expression. Additionally, T-CD8 + but no T-CD4 + cells showed a suppressive phenotype with increased Tim-3 and PD1 expression in WT and IL-4Rα -/- , which were absent in T. crassiceps -infected LysMcreIL-4Rα -/lox mice. These findings demonstrate a critical role for the IL-4 signaling pathway in sustaining AAM and its suppressive activity during cysticercosis, suggesting a pivotal role for AAM in favoring susceptibility to T. crassiceps infection. Thus, the absence of these suppressor cells is one of the leading mechanisms to control experimental cysticercosis successfully. |
