Cell Surface Parameters for Accessing Neutrophil Activation Level with Atomic Force Microscopy.

Autor: Tilinova OM; Sarov Physics and Technology Institute, MEPhI, 607186 Sarov, Russia., Inozemtsev V; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Sherstyukova E; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Kandrashina S; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Pisarev M; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Grechko A; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Vorobjeva N; Department of Immunology, Biology Faculty, Lomonosov Moscow State University, 119234 Moscow, Russia., Sergunova V; Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, Russia., Dokukin ME; Sarov Physics and Technology Institute, MEPhI, 607186 Sarov, Russia.
Jazyk: angličtina
Zdroj: Cells [Cells] 2024 Feb 07; Vol. 13 (4). Date of Electronic Publication: 2024 Feb 07.
DOI: 10.3390/cells13040306
Abstrakt: In this study, we examine the topography and adhesion images of the cell surface of neutrophils during the activation process. Our analysis of cell surface parameters indicates that the most significant changes in neutrophils occur within the first 30 min of activation, suggesting that reactive oxygen species may require approximately this amount of time to activate the cells. Interestingly, we observed surface granular structure as early as 10 min after neutrophil activation when examining atomic force microscopy images. This finding aligns with the reorganization observed within the cells under confocal laser scanning microscopy. By analyzing the cell surface images of adhesion, we identified three spatial surface parameters that correlate with the activation time. This finding enables us to estimate the degree of activation by using atomic force microscopy maps of the cell surface.
Databáze: MEDLINE
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