Advanced Platelet Lysate Aerogels: Biomaterials for Regenerative Applications.

Autor:	Tibourtine F; CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, CEDEX 9, 31062 Toulouse, France., Canceill T; Département Odontologie, Faculté de Santé, Hôpitaux de Toulouse, Université Paul Sabatier, 3 Chemin des Maraichers, CEDEX 9, 31062 Toulouse, France., Marfoglia A; CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, CEDEX 9, 31062 Toulouse, France.; Laboratoire de Génie Chimique, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, France., Lavalle P; Institut National de la Santé et de la Recherche Médicale, Inserm UMR_S 1121 Biomaterials and Bioengineering, 67085 Strasbourg, France., Gibot L; Laboratoire Softmat, Université de Toulouse, CNRS UMR 5623, Université Toulouse III-Paul Sabatier, 31062 Toulouse, France., Pilloux L; Laboratoire de Génie Chimique, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, France., Aubry C; ARNA, Inserm U1212, CNRS 5320, University of Bordeaux, 146 Rue Léo Saignat, CEDEX, 33076 Bordeaux, France., Medemblik C; Institut National de la Santé et de la Recherche Médicale, Inserm UMR_S 1121 Biomaterials and Bioengineering, 67085 Strasbourg, France., Goudouneche D; Centre de Microscopie Electronique Appliquée à la Biologie, Faculté de Médecine, 133 Route de Narbonne, 31062 Toulouse, France., Dupret-Bories A; CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, CEDEX 9, 31062 Toulouse, France.; Department of Surgery, University Cancer Institute of Toulouse-Oncopole, 1 Avenue Irène Joliot-Curie, 31100 Toulouse, France.; Department of Ear, Nose and Throat Surgery, Toulouse University Hospital-Larrey Hospital, 31400 Toulouse, France., Cazalbou S; CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 118 Route de Narbonne, CEDEX 9, 31062 Toulouse, France.
Jazyk:	angličtina
Zdroj:	Journal of functional biomaterials [J Funct Biomater] 2024 Feb 19; Vol. 15 (2). Date of Electronic Publication: 2024 Feb 19.
DOI:	10.3390/jfb15020049
Abstrakt:	Human platelet lysate (HPL), rich in growth factors, is increasingly recognized for its potential in tissue engineering and regenerative medicine. However, its use in liquid or gel form is constrained by limited stability and handling difficulties. This study aimed to develop dry and porous aerogels from HPL hydrogel using an environmentally friendly supercritical CO 2 -based shaping process, specifically tailored for tissue engineering applications. The aerogels produced retained their three-dimensional structure and demonstrated significant mechanical robustness and enhanced manageability. Impressively, they exhibited high water absorption capacity, absorbing 87% of their weight in water within 120 min. Furthermore, the growth factors released by these aerogels showed a sustained and favourable biological response in vitro. They maintained the cellular metabolic activity of fibroblasts (BALB-3T3) at levels akin to conventional culture conditions, even after prolonged storage, and facilitated the migration of human umbilical vein endothelial cells (HUVECs). Additionally, the aerogels themselves supported the adhesion and proliferation of murine fibroblasts (BALB-3T3). Beyond serving as excellent matrices for cell culture, these aerogels function as efficient systems for the delivery of growth factors. Their multifunctional capabilities position them as promising candidates for various tissue regeneration strategies. Importantly, the developed aerogels can be stored conveniently and are considered ready to use, enhancing their practicality and applicability in regenerative medicine.
Databáze:	MEDLINE
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