Impact of age on pneumococcal colonization of the nasopharynx and oral cavity: an ecological perspective.

Autor:	Miellet WR; Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht (UMCU), Wilhelmina Children's Hospital, Utrecht, 3584 CX, The Netherlands.; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Mariman R; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., van Veldhuizen J; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Badoux P; Regional Laboratory of Public Health (Streeklab) Haarlem, Haarlem, 2035 RC, The Netherlands., Wijmenga-Monsuur AJ; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Litt D; Respiratory and Vaccine Preventable Bacterial Reference Unit (RVPBRU) and Immunisation and Vaccine Preventable Diseases Division, UK Health Security Agency, London, NW9 5EQ, United Kingdom., Bosch T; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Miller E; School of Hygiene and Tropical Medicine, Department of Infectious Disease Epidemiology, London, WC1E 7HT, United Kingdom., Fry NK; Respiratory and Vaccine Preventable Bacterial Reference Unit (RVPBRU) and Immunisation and Vaccine Preventable Diseases Division, UK Health Security Agency, London, NW9 5EQ, United Kingdom., van Houten MA; Department of Pediatrics, Spaarne Gasthuis, Haarlem, 2035 RC, The Netherlands., Rots NY; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Sanders EAM; Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht (UMCU), Wilhelmina Children's Hospital, Utrecht, 3584 CX, The Netherlands.; Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, 3721 MA, The Netherlands., Trzciński K; Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht (UMCU), Wilhelmina Children's Hospital, Utrecht, 3584 CX, The Netherlands.
Jazyk:	angličtina
Zdroj:	ISME communications [ISME Commun] 2024 Jan 12; Vol. 4 (1), pp. ycae002. Date of Electronic Publication: 2024 Jan 12 (Print Publication: 2024).
DOI:	10.1093/ismeco/ycae002
Abstrakt:	Pneumococcal carriage studies have suggested that pneumococcal colonization in adults is largely limited to the oral cavity and oropharynx. In this study, we used total abundance-based β-diversity (dissimilarity) and β-diversity components to characterize age-related differences in pneumococcal serotype composition of respiratory samples. quantitative PCR (qPCR) was applied to detect pneumococcal serotypes in nasopharyngeal samples collected from 946 toddlers and 602 adults, saliva samples collected from a subset of 653 toddlers, and saliva and oropharyngeal samples collected from a subset of 318 adults. Bacterial culture rates from nasopharyngeal samples were used to characterize age-related differences in rates of colonizing bacteria. Dissimilarity in pneumococcal serotype composition was low among saliva and nasopharyngeal samples from children. In contrast, respiratory samples from adults exhibited high serotype dissimilarity, which predominantly consisted of abundance gradients and was associated with reduced nasopharyngeal colonization. Age-related serotype dissimilarity was high among nasopharyngeal samples and relatively low for saliva samples. Reduced nasopharyngeal colonization by pneumococcal serotypes coincided with significantly reduced Moraxella catarrhalis and Haemophilus influenzae and increased Staphylococcus aureus nasopharyngeal colonization rates among adults. Findings from this study suggest that within-host environmental conditions, utilized in the upper airways by pneumococcus and other bacteria, undergo age-related changes. It may result in a host-driven ecological succession of bacterial species colonizing the nasopharynx and lead to competitive exclusion of pneumococcus from the nasopharynx but not from the oral habitat. This explains the poor performance of nasopharyngeal samples for pneumococcal carriage among adults and indicates that in adults saliva more accurately represents the epidemiology of pneumococcal carriage than nasopharyngeal samples. Competing Interests: UKHSA provides vaccine manufacturers (GSK, Pfizer, and Sanofi) with postmarketing surveillance reports on pneumococcal infection which the companies are required to submit to the UK licensing authority in compliance with their Risk Management Strategy. A cost recovery charge is made for these reports. UKHSA has received unrestricted research grants from Pfizer to participate in pneumococcal surveillance projects. K.T. received funds for an unrestricted research grant from GlaxoSmithKline Biologicals SA, consultation fees, fees for participation in advisory boards, speaking fees, and funds for unrestricted research grants from Pfizer, funds for an unrestricted research grant, and fees for participating in advisory boards from Merck Sharp & Dohme, all paid directly to his home institution. Except for the funds from GlaxoSmithKline Biologicals SA, none was received in relation to the work reported here. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© The Author(s) [2024]. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu