Acid-active proteases to optimize dietary protein digestibility: a step towards sustainable nutrition.

Autor:	Mak WS; Department of Chemistry, University of California, Davis, Davis, CA, United States.; Digestiva, Inc., Davis, CA, United States., Jones CP; Digestiva, Inc., Davis, CA, United States., McBride KE; Digestiva, Inc., Davis, CA, United States., Fritz EAP; Empowered Science, LLC, South Barrington, IL, United States., Hirsch J; Digestiva, Inc., Davis, CA, United States., German JB; Department of Food Science and Technology, University of California, Davis, Davis, CA, United States., Siegel JB; Department of Chemistry, University of California, Davis, Davis, CA, United States.; Digestiva, Inc., Davis, CA, United States.; Genome Center, University of California, Davis, Davis, CA, United States.; Department of Biochemistry and Molecular Medicine, University of California, Davis, Davis, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in nutrition [Front Nutr] 2024 Feb 08; Vol. 11, pp. 1291685. Date of Electronic Publication: 2024 Feb 08 (Print Publication: 2024).
DOI:	10.3389/fnut.2024.1291685
Abstrakt:	Introduction: Historically, prioritizing abundant food production often resulted in overlooking nutrient quality and bioavailability, however, environmental concerns have now propelled sustainable nutrition and health efficacy to the forefront of global attention. In fact, increasing demand for protein is the major challenge facing the food system in the 21st century with an estimation that 70% more food is needed by 2050. This shift has spurred interest in plant-based proteins for their sustainability and health benefits, but most alternative sources of protein are poorly digestible. There are two approaches to solve digestibility: improve the digestibility of food proteins or improve the digestive capacity of consumers. Enhancing nutrient digestibility and bioavailability across diverse protein sources is crucial, with proteases presenting a promising avenue. Research, inspired by the proteases of human breast milk, has demonstrated that exogenous microbial proteases can activate within the human digestive tract and substantially increase the digestion of targeted proteins that are otherwise difficult to fully digest. Methods: Here, we introduce the use of an acid-active family of bacterial proteases (S53) to improve the digestibility and nutritional quality of a variety of protein sources, evaluated using the INFOGEST 2.0 protocol. Results: Results from in vitro digestibility indicate that the most effective protease in the S53 family substantially improves the digestibility of an array of animal and plant-derived proteins-soy, pea, chickpea, rice, casein, and whey. On average, this protease elevated protein digestibility by 115% during the gastric phase and by 15% in the intestinal phase, based on the degree of hydrolysis. Discussion: The widespread adoption of these proteases has the potential to enhance nutritional value and contribute to food security and sustainability. This approach would complement ongoing efforts to improve proteins in the food supply, increase the quality of more sustainable protein sources and aid in the nourishment of patients with clinically compromised, fragile intestines and individuals like older adults and high-performance athletes who have elevated protein needs. Competing Interests: WM, CJ, KM, and JH are employed by Digestiva, Inc. This study was funded in part by Mars Inc. and Digestiva, Inc. The funder, Mars Inc. was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication the funder, Digestiva, Inc. paid for the time and effort of researchers to collect data published herein. EF has ownership in Empowered Science, LLC. JG and JS are employees of University of California, Davis and scientific advisors to Digestiva, Inc. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Mak, Jones, McBride, Fritz, Hirsch, German and Siegel.)
Databáze:	MEDLINE
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