Fine needle aspiration of human lymph nodes reveals cell populations and soluble interactors pivotal to immunological priming.

Autor:	Provine NM; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Al-Diwani A; Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.; University Department of Psychiatry, University of Oxford, Oxford, UK., Agarwal D; Wellcome Centre for Human Genetics, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Dooley K; Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Heslington A; Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Murchison AG; Department of Radiology, Oxford University Hospitals NHS Foundation Trust, Oxford, UK., Garner LC; Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, Oxford, UK., Sheerin F; Department of Radiology, Oxford University Hospitals NHS Foundation Trust, Oxford, UK., Klenerman P; Pandemic Sciences Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.; Translational Gastroenterology Unit, Nuffield Department of Medicine, University of Oxford, Oxford, UK.; Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, University of Oxford, Oxford, Oxfordshire, UK., Irani SR; Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, UK.; Department of Neurology, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
Jazyk:	angličtina
Zdroj:	European journal of immunology [Eur J Immunol] 2024 May; Vol. 54 (5), pp. e2350872. Date of Electronic Publication: 2024 Feb 22.
DOI:	10.1002/eji.202350872
Abstrakt:	Lymph node (LN) fine needle aspiration (LN FNA) represents a powerful technique for minimally invasive sampling of human LNs in vivo and has been used effectively to directly study aspects of the human germinal center response. However, systematic deep phenotyping of the cellular populations and cell-free proteins recovered by LN FNA has not been performed. Thus, we studied human cervical LN FNAs as a proof-of-concept and used single-cell RNA-sequencing and proteomic analysis to benchmark this compartment, define the purity of LN FNA material, and facilitate future studies in this immunologically pivotal environment. Our data provide evidence that LN FNAs contain bone-fide LN-resident innate immune populations, with minimal contamination of blood material. Examination of these populations reveals unique biology not predictable from equivalent blood-derived populations. LN FNA supernatants represent a specific source of lymph- and lymph node-derived proteins, and can, aided by transcriptomics, identify likely receptor-ligand interactions. This represents the first description of the types and abundance of immune cell populations and cell-free proteins that can be efficiently studied by LN FNA. These findings are of broad utility for understanding LN physiology in health and disease, including infectious or autoimmune perturbations, and in the case of cervical nodes, neuroscience. (© 2024 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH.)
Databáze:	MEDLINE
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