An Open-Source Mouse Chronic EEG Array System with High-Density MXene-Based Skull Surface Electrodes.

Autor: Ding L; Department of Neurology, Vanderbilt University School of Medicine, Nashville 37232, Tennessee., Patel A; Department of Neurology, Vanderbilt University School of Medicine, Nashville 37232, Tennessee., Shankar S; Departments of Bioengineering and Neurology, Center for Neuroengineering & Therapeutics, University of Pennsylvania, Philadelphia 19104, Pennsylvania., Driscoll N; Departments of Bioengineering and Neurology, Center for Neuroengineering & Therapeutics, University of Pennsylvania, Philadelphia 19104, Pennsylvania., Zhou C; Department of Neurology, Vanderbilt University School of Medicine, Nashville 37232, Tennessee., Rex TS; Department of Ophthalmology & Visual Sciences, Vanderbilt University School of Medicine, Nashville 37232, Tennessee., Vitale F; Departments of Bioengineering and Neurology, Center for Neuroengineering & Therapeutics, University of Pennsylvania, Philadelphia 19104, Pennsylvania.; Center for Neurotrauma, Neurodegeneration, and Restoration, Corporal Michael J. Crescenz Veterans Affairs Medical Center, Philadelphia 19104, Pennsylvania., Gallagher MJ; Department of Neurology, Vanderbilt University School of Medicine, Nashville 37232, Tennessee martin.j.p.gallagher@gmail.com.; Department of Veteran's Affairs, Tennessee Valley Health System, Nashville 37212, Tennessee.
Jazyk: angličtina
Zdroj: ENeuro [eNeuro] 2024 Feb 22; Vol. 11 (2). Date of Electronic Publication: 2024 Feb 22 (Print Publication: 2024).
DOI: 10.1523/ENEURO.0512-22.2023
Abstrakt: Electroencephalography (EEG) is an indispensable tool in epilepsy, sleep, and behavioral research. In rodents, EEG recordings are typically performed with metal electrodes that traverse the skull into the epidural space. In addition to requiring major surgery, intracranial EEG is difficult to perform for more than a few electrodes, is time-intensive, and confounds experiments studying traumatic brain injury. Here, we describe an open-source cost-effective refinement of this technique for chronic mouse EEG recording. Our alternative two-channel (EEG2) and sixteen-channel high-density EEG (HdEEG) arrays use electrodes made of the novel, flexible 2D nanomaterial titanium carbide (Ti 3 C 2 T x ) MXene. The MXene electrodes are placed on the surface of the intact skull and establish an electrical connection without conductive gel or paste. Fabrication and implantation times of MXene EEG electrodes are significantly shorter than the standard approach, and recorded resting baseline and epileptiform EEG waveforms are similar to those obtained with traditional epidural electrodes. Applying HdEEG to a mild traumatic brain injury (mTBI) model in mice of both sexes revealed that mTBI significantly increased spike-wave discharge (SWD) preictal network connectivity with frequencies of interest in the β-spectral band (12-30 Hz). These findings indicate that the fabrication of MXene electrode arrays is a cost-effective, efficient technology for multichannel EEG recording in mice that obviates the need for skull-penetrating surgery. Moreover, increased preictal β-frequency network connectivity may contribute to the development of early post-mTBI SWDs.
(Copyright © 2024 Ding et al.)
Databáze: MEDLINE
Externí odkaz: