Effective plant virus enrichment using carbon nanotubes and microfluidics.

Autor: Perea Lopez N; Department of Physics, The Pennsylvania State University, University Park, PA, 16802, USA; Center for 2-Dimensional and Layered Materials, The Pennsylvania State University, University Park, PA, 16802, USA., Iturralde Martinez JF; Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, 16802, USA., Vosburg C; Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, 16802, USA., Rajotte EG; Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, 16802, USA; Entomology, The Pennsylvania State University, 501 ASI, University Park, State College, PA, 16802, USA., Rosa C; Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA, 16802, USA., Terrones M; Department of Physics, The Pennsylvania State University, University Park, PA, 16802, USA; Center for 2-Dimensional and Layered Materials, The Pennsylvania State University, University Park, PA, 16802, USA; Department of Chemistry, The Pennsylvania State University, University Park, PA, 16802, USA; Department of Materials Science and Engineering, The Pennsylvania State University, University Park, Pennsylvania, 16802, USA; Research Initiative for Supra Materials, Shinshu University, Nagano 380-8553, Japan. Electronic address: mut11@psu.edu.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 May; Vol. 326, pp. 114905. Date of Electronic Publication: 2024 Feb 22.
DOI: 10.1016/j.jviromet.2024.114905
Abstrakt: Plant virus detection and identification in crops is a pillar for disease management, import of crop material, production of clean stock plants and basic plant virology studies. In this report, we present a platform for the enrichment and isolation of known or unknown viruses. This platform is based on carbon nanotube arrays inside a microfluidic device that can be a solution for the identification of low titer viruses from plants. Using our microfluidic devices, we achieved enrichment of two economically important viruses, the orthotospovirus, tomato spotted wilt orthotospovirus (TSWV) and the potyvirus, zucchini yellow mosaic virus (ZYMV). The carbon nanotube arrays integrated in these microfluidic devices are capable of trapping viruses discriminated by their size; the virus rich arrays can be then analyzed by common downstream techniques including immunoassays, PCR, HTS and electron microscopy. This procedure offers a simple to operate and portable sample preparation device capable of trapping viruses from raw plant extracts while reducing the host contamination.
Competing Interests: Declaration of Competing Interest M.T. and N.P.L. hold equity in Virolock Technologies LLC; a company engaged in the commercialization of CNTMDs. C. R., F. I., C. V., and E. R. declare that they have no known competing financial interests or personal relationships that could have influenced the research reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: