Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish Hypostomus albopunctatus (Siluriformes: Loricariidae) Revealed by Heterochromatin Accumulation.

Autor:	Balini LC; Department of Biotechnology, Genetics, and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil., Fernandes CA; Department of Biotechnology, Genetics, and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.; Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maringá, Maringá, Paraná, Brazil., Portela-Castro ALB; Department of Biotechnology, Genetics, and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.; Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maringá, Maringá, Paraná, Brazil., Melo RF; Department of Biotechnology, Genetics, and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil., Zawadzki CH; Limnology, Ichthyology and Aquaculture Research Nucleus (NUPELIA), Biological Sciences Center, State University of Maringá, Maringá, Paraná, Brazil.; Department of Biology, State University of Maringá, Maringá, Paraná, Brazil., Borin-Carvalho LA; Department of Biotechnology, Genetics, and Cell Biology, State University of Maringá, Maringá, Paraná, Brazil.
Jazyk:	angličtina
Zdroj:	Zebrafish [Zebrafish] 2024 Jun; Vol. 21 (3), pp. 265-273. Date of Electronic Publication: 2024 Feb 20.
DOI:	10.1089/zeb.2023.0100
Abstrakt:	In fish species, heterochromatinization is one process that could trigger sex chromosome differentiation. The present article describes a nascent XX/XY sex chromosome system evidenced by heterochromatin accumulation and microsatellite (GATA) 8 in Hypostomus albopunctatus from two populations of the Paraná River basin. The specimens of H. albopunctatus from the Campo and Bossi Rivers share the same karyotype. The species exhibits 74 chromosomes (8m+14sm +16st +36a, fundamental number = 112). The C-banding technique suggests male heterogamety in H. albopunctatus , where the Y-chromosome is morphologically like the X-chromosome but differs from it for having long arms that are entirely heterochromatic. Double fluorescence in situ hybridization (FISH) with 18S and 5S rDNA probes confirmed the Ag-nucleolus organizer region sites in a single pair for both populations, and minor rDNA clusters showed interpopulational variation. FISH with the microsatellite (GATA) 8 probe showed a dispersed pattern in the karyotype, accumulating these sequences of sex chromosomes of both populations. FISH with microsatellite (CGC) 10 probe showed interpopulational variation. The absence of differentiated sex chromosomes in H. albopunctatus is described previously, and a new variant is documented herein where XY chromosomes can be seen in an early stage of differentiation.
Databáze:	MEDLINE
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