Development and characterization of a new sunflower source of resistance to race G of Orobanche cumana Wallr. derived from Helianthus anomalus.

Autor: Fernández-Melero B; Instituto de Agricultura Sostenible (IAS-CSIC), Alameda del Obispo S/N, 14004, Córdoba, Spain., Del Moral L; Instituto de Agricultura Sostenible (IAS-CSIC), Alameda del Obispo S/N, 14004, Córdoba, Spain., Todesco M; Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.; Biodiversity Research Centre, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada., Rieseberg LH; Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.; Biodiversity Research Centre, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada., Owens GL; Department of Biology, University of Victoria, Victoria, BC, V8W 2Y2, Canada., Carrère S; Laboratoire des Interactions Plantes Microbes-Environnement (LIPME), Université de Toulouse, CNRS, INRAE, Castanet-Tolosan, France., Chabaud M; Laboratoire des Interactions Plantes Microbes-Environnement (LIPME), Université de Toulouse, CNRS, INRAE, Castanet-Tolosan, France., Muños S; Laboratoire des Interactions Plantes Microbes-Environnement (LIPME), Université de Toulouse, CNRS, INRAE, Castanet-Tolosan, France., Velasco L; Instituto de Agricultura Sostenible (IAS-CSIC), Alameda del Obispo S/N, 14004, Córdoba, Spain., Pérez-Vich B; Instituto de Agricultura Sostenible (IAS-CSIC), Alameda del Obispo S/N, 14004, Córdoba, Spain. bperez@ias.csic.es.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Feb 22; Vol. 137 (3), pp. 56. Date of Electronic Publication: 2024 Feb 22.
DOI: 10.1007/s00122-024-04558-4
Abstrakt: Key Message: A new Or Anom1 gene introgressed in cultivated sunflower from wild Helianthus anomalus confers late post-attachment resistance to Orobanche cumana race G and maps to a target interval in Chromosome 4 where two receptor-like kinases (RLKs) have been identified in the H. anomalus genome as putative candidates. Sunflower broomrape is a parasitic weed that infects sunflower (Helianthus annuus L.) roots causing severe yield losses. Breeding for resistance is the most effective and sustainable control method. In this study, we report the identification, introgression, and genetic and physiological characterization of a new sunflower source of resistance to race G of broomrape developed from the wild annual sunflower H. anomalus (accession PI 468642). Crosses between PI 468642 and the susceptible line P21 were carried out, and the genetic study was conducted in BC 1 F 1 , BC 1 F 2, and its derived BC 1 F 3 populations. A BC 1 F 5 germplasm named ANOM1 was developed through selection for race G resistance and resemblance to cultivated sunflower. The resistant trait showed monogenic and dominant inheritance. The gene, named Or Anom1 , was mapped to Chromosome 4 within a 1.2 cM interval and co-segregated with 7 SNP markers. This interval corresponds to a 1.32 Mb region in the sunflower reference genome, housing a cluster of receptor-like kinase and receptor-like protein (RLK-RLP) genes. Notably, the analysis of the H. anomalus genome revealed the absence of RLPs in the Or Anom1 target region but featured two RLKs as possible Or Anom1 candidates. Rhizotron and histological studies showed that Or Anom1 determines a late post-attachment resistance mechanism. Broomrape can establish a vascular connection with the host, but parasite growth is stopped before tubercle development, showing phenolic compounds accumulation and tubercle necrosis. ANOM1 will contribute to broadening the genetic basis of broomrape resistance in the cultivated sunflower pool and to a better understanding of the molecular basis of the sunflower-broomrape interaction.
(© 2024. The Author(s).)
Databáze: MEDLINE
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