[Effects of upstream laboratory processes on the digitization of histological slides].

Autor: Schwaibold L; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland., Mattern S; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland., Mählmann M; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland., Lobert L; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland., Breunig T; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland., Schürch CM; Institut für Pathologie, Universitätsklinikum Tübingen, Liebermeisterstr. 8, 72076, Tübingen, Deutschland. christian.schuerch@med.uni-tuebingen.de.
Jazyk: němčina
Zdroj: Pathologie (Heidelberg, Germany) [Pathologie (Heidelb)] 2024 Mar; Vol. 45 (2), pp. 90-97. Date of Electronic Publication: 2024 Feb 22.
DOI: 10.1007/s00292-024-01303-y
Abstrakt: Background: Several factors in glass slide (GS) preparation affect the quality and data volume of a digitized histological slide. In particular, reducing contamination and selecting the appropriate coverslip have the potential to significantly reduce scan time and data volume.
Goals: To objectify observations from our institute's digitization process to determine the impact of laboratory processes on the quality of digital histology slides.
Materials and Methods: Experiment 1: Scanning the GS before and after installation of a central console in the microtomy area to reduce dirt and statistical analysis of the determined parameters. Experiment 2: Re-coverslipping the GS (post diagnostics) with glass and film. Scanning the GS and statistical analysis of the collected parameters.
Conclusion: The targeted restructuring in the laboratory process leads to a reduction of GS contamination. This causes a significant reduction in the amount of data generated and scanning time required for the digitized sections. Film as a coverslip material minimizes processing errors in contrast to glass. According to our estimation, all the above-mentioned points lead to considerable cost savings.
(© 2024. The Author(s).)
Databáze: MEDLINE
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