Bimodal distribution of azole susceptibility in Sporothrix brasiliensis isolates in Brazil.

Autor:	Ribeiro Dos Santos A; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Gade L; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Misas E; Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA.; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Litvintseva AP; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Nunnally NS; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Parnell LA; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Rajeev M; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., de Souza Carvalho Melhem M; School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.; Graduate Program in Tropical Diseases, Universidade Estadual Paulista, Botucatu, SP, Brazil.; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil., Takahashi JPF; School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil., Oliboni GM; Graduate Program in Sciences, Coordenadoria de Controle de Doenças, Secretary of Health, São Paulo, Brazil., Bonfieti LX; Parasitology and Mycology Center, Instituto Adolfo Lutz, São Paulo, SP, Brazil., Araujo LS; Central Public Health Laboratory of Mato Grosso do Sul, Secretary of Health, Campo Grande, MS, Brazil., Cappellano P; Microbiology Section, Grupo Fleury, São Paulo, SP, Brazil., Venturini J; School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil., Lockhart SR; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA., Sexton DJ; Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Jazyk:	angličtina
Zdroj:	Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2024 Apr 03; Vol. 68 (4), pp. e0162023. Date of Electronic Publication: 2024 Feb 22.
DOI:	10.1128/aac.01620-23
Abstrakt:	Sporothrix brasiliensis is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample of 61 Sporothrix spp. isolates from human and cat sporotrichosis cases in Brazil using the Clinical and Laboratory Standards Institute standard M38. A bimodal distribution of azole susceptibility was observed with 50% (28/56) of S. brasiliensis isolates showing elevated itraconazole minimum inhibitory concentrations ≥16 µg/mL. Phylogenetic analysis found the in vitro resistant isolates were not clonal and were distributed across three different S. brasiliensis clades. Single nucleotide polymorphism (SNP) analysis was performed to identify potential mechanisms of in vitro resistance. Two of the 28 resistant isolates (MIC ≥16 mg/L) had a polymorphism in the cytochrome P450 gene, cyp51 , corresponding to the well-known G448S substitution inducing azole resistance in Aspergillus fumigatus . SNPs corresponding to other known mechanisms of azole resistance were not identified in the remaining 26 in vitro resistant isolates. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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