Time-efficient germ cell transplantation from goldfish ( Carassius auratus ) into adult common carp ( Cyprinus carpio ).

Autor:	Vigoya AAA; Centro de Aquicultura, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.; Facultad de Medicina Veterinaria y Zootecnia, Fundación Universitaria San Martín, Bogotá, Colombia., da Costa DF; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., de Oliveira MA; Centro de Aquicultura, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Butzge AJ; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Rosa IF; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Doretto LB; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil.; National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China., Martinez ERM; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Digmayer M; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil., Nóbrega RH; Departamento de Biologia Estrutural e Funcional, Instituto de Biosciências, Universidade Estadual Paulista, Botucatu, SP, Brasil.
Jazyk:	angličtina
Zdroj:	Animal reproduction [Anim Reprod] 2024 Feb 12; Vol. 21 (1), pp. e20230121. Date of Electronic Publication: 2024 Feb 12 (Print Publication: 2024).
DOI:	10.1590/1984-3143-AR2023-0121
Abstrakt:	Germ cell transplantation in fish is a promising technique for surrogate broodstock parents with broader application in aquaculture and conserving endangered and valuable genetic resources. Herein, we describe the establishment of an intrapapillary xenogeneic transplant of germ cells from sexually mature goldfish ( C. auratus ) males into common carp ( C. carpio ) males cytoablated with a thermochemical treatment (two doses of busulfan at 40 mg/kg at 35°C). To analyze the presence and development of donor germ cells in recipient testes, donor germ cells were labeled with PKH26, a fluorescent cell membrane dye, before transplantation. Our results demonstrated that thermochemical treatment caused effective spermatogenesis suppression and pronounced germ cell loss. Moreover, transplanted spermatogonial cells were able to colonize the recipients' testes, resume spermatogenesis, and generate spermatozoa within eight weeks after germ cell transplantation. These findings suggested that recipient testes provided suitable conditions for the survival, colonization, proliferation, and differentiation of donor spermatogonia from a related species. This study indicated that recipients' testes exhibited a high degree of plasticity to accept and support xenogeneic donor germ cells, which were able to form sperm in a short time frame. This approach has significant implications for assisted animal reproduction, biotechnology, conservation, and the production of valuable genetic resources and endangered fish species. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare.
Databáze:	MEDLINE
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