UV-C LED-induced cyclobutane pyrimidine dimer formation, lesion repair and mutagenesis in the biofilm-forming diatom, Navicula incerta .

Autor: Whitworth P; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom., Aldred N; School of Life Sciences, University of Essex, Colchester, United Kingdom., Finlay JA; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom., Reynolds KJ; Technology & Innovation Delivery, Marine, Protective and Yacht, AkzoNobel/International Paint Ltd, Felling, Gateshead, United Kingdom., Plummer J; Physical Sciences Group, Platform Systems Division, Defence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, United Kingdom., Clare AS; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.
Jazyk: angličtina
Zdroj: Biofouling [Biofouling] 2024 Jan; Vol. 40 (1), pp. 76-87. Date of Electronic Publication: 2024 Feb 21.
DOI: 10.1080/08927014.2024.2319178
Abstrakt: The use of ultraviolet-C (UV-C) irradiation in marine biofouling control is a relatively new and potentially disruptive technology. This study examined effects of UV-C exposure on the biofilm-forming diatom, Navicula incerta . UV-C-induced mutations were identified via Illumina HiSeq. A de novo genome was assembled from control sequences and reads from UV-C-exposed treatments were mapped to this genome, with a quantitative estimate of mutagenesis then derived from the frequency of single nucleotide polymorphisms. UV-C exposure increased cyclobutane pyrimidine dimer (CPD) abundance with a direct correlation between lesion formation and fluency. Cellular repair mechanisms gradually reduced CPDs over time, with the highest UV-C fluence treatments having the fastest repair rates. Mutation abundances were, however, negatively correlated with CPD abundance suggesting that UV-C exposure may influence lesion repair. The threshold fluence for CPD formation exceeding CPD repair was >1.27 J cm -2 . Fluences >2.54 J cm -2 were predicted to inhibit repair mechanisms. While UV-C holds considerable promise for marine antifouling, diatoms are just one, albeit an important, component of marine biofouling communities. Determining fluence thresholds for other representative taxa, highlighting the most resistant, would allow UV-C treatments to be specifically tuned to target biofouling organisms, whilst limiting environmental effects and the power requirement.
Databáze: MEDLINE