Regulation of myocardial contraction as revealed by intracellular Ca 2+ measurements using aequorin.

Autor: Kurihara S; Department of Cell Physiology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-Ku, Tokyo, 105-8461, Japan. kurihara@jikei.ac.jp., Fukuda N; Department of Cell Physiology, The Jikei University School of Medicine, 3-25-8 Nishi-Shimbashi, Minato-Ku, Tokyo, 105-8461, Japan.
Jazyk: angličtina
Zdroj: The journal of physiological sciences : JPS [J Physiol Sci] 2024 Feb 21; Vol. 74 (1), pp. 12. Date of Electronic Publication: 2024 Feb 21.
DOI: 10.1186/s12576-024-00906-7
Abstrakt: Of the ions involved in myocardial function, Ca 2+ is the most important. Ca 2+ is crucial to the process that allows myocardium to repeatedly contract and relax in a well-organized fashion; it is the process called excitation-contraction coupling. In order, therefore, for accurate comprehension of the physiology of the heart, it is fundamentally important to understand the detailed mechanism by which the intracellular Ca 2+ concentration is regulated to elicit excitation-contraction coupling. Aequorin was discovered by Shimomura, Johnson and Saiga in 1962. By taking advantage of the fact that aequorin emits blue light when it binds to Ca 2+ within the physiologically relevant concentration range, in the 1970s and 1980s, physiologists microinjected it into myocardial preparations. By doing so, they proved that Ca 2+ transients occur upon membrane depolarization, and tension development (i.e., actomyosin interaction) subsequently follows, dramatically advancing the research on cardiac excitation-contraction coupling.
(© 2024. The Author(s).)
Databáze: MEDLINE
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