Shift in hemocyte immune parameters of marine bivalve Mytilus galloprovincialis (Lamarck, 1819) after exposure to methane.

Autor: Kladchenko ES; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia. Electronic address: Kladchenko_ekaterina@bk.ru., Chelebieva ES; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia., Podolskaya MS; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia., Khurchak AI; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia; Department of Radiation and Chemical Biology, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia., Andreyeva AY; Laboratory of Ecological Immunology of Aquatic Organisms, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia., Malakhova TV; Department of Radiation and Chemical Biology, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Leninsky ave, 14, Moscow 119991, Russia.
Jazyk: angličtina
Zdroj: Marine pollution bulletin [Mar Pollut Bull] 2024 Apr; Vol. 201, pp. 116174. Date of Electronic Publication: 2024 Feb 21.
DOI: 10.1016/j.marpolbul.2024.116174
Abstrakt: Methane are widely used in industry as an emerge source may be released significantly higher aquatic ecosystems due to gas seepages. In this study, short-term (90 min) methane effects on bivalve hemocytes were investigated using flow cytometry. Hemocyte parameters including hemolymph cellular composition, phagocytosis activity, mitochondrial membrane potential and reactive oxygen species (ROS) content were evaluated in the mussel Mytilus galloprovincialis (Lamarck, 1819) exposed to hypoxia (control group), pure methane and industrial methane (industrial hydrocarbon mixture). Comparison of biomarkers showed that the mussel was more sensitive to methane than to low oxygen concentration, supporting the effects of methane on the mussel's immune system. After exposure to pure and industrial methane, the number of granulocytes decreased dramatically and the levels of reactive oxygen species, mitochondrial membrane potential and phagocytosis capacity increased significantly. It was shown that the methane type-dependent effect was pronounced, with industrial methane leading to more pronounced changes.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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