The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man.
| Autor: | Adiji OA; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA., McConnell BS; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA., Parker MW; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Nucleic acids research [Nucleic Acids Res] 2024 May 08; Vol. 52 (8), pp. 4344-4360. |
| DOI: | 10.1093/nar/gkae122 |
| Abstrakt: | The first step toward eukaryotic genome duplication is loading of the replicative helicase onto chromatin. This 'licensing' step initiates with the recruitment of the origin recognition complex (ORC) to chromatin, which is thought to occur via ORC's ATP-dependent DNA binding and encirclement activity. However, we have previously shown that ATP binding is dispensable for the chromatin recruitment of fly ORC, raising the question of how metazoan ORC binds chromosomes. We show here that the intrinsically disordered region (IDR) of fly Orc1 is both necessary and sufficient for recruitment of ORC to chromosomes in vivo and demonstrate that this is regulated by IDR phosphorylation. Consistently, we find that the IDR confers the ORC holocomplex with ATP-independent DNA binding activity in vitro. Using phylogenetic analysis, we make the surprising observation that metazoan Orc1 IDRs have diverged so markedly that they are unrecognizable as orthologs and yet we find that these compositionally homologous sequences are functionally conserved. Altogether, these data suggest that chromatin is recalcitrant to ORC's ATP-dependent DNA binding activity, necessitating IDR-dependent chromatin tethering, which we propose poises ORC to opportunistically encircle nucleosome-free regions as they become available. (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
| Databáze: | MEDLINE |
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