| Autor: |
Chibli LA; Green Biotech Network, School of Agricultural Sciences, São Paulo State University (UNESP), 18610-034 Botucatu, Brazil., de Lima BR; Green Biotech Network, School of Agricultural Sciences, São Paulo State University (UNESP), 18610-034 Botucatu, Brazil., Carneiro AM; Green Biotech Network, School of Agricultural Sciences, São Paulo State University (UNESP), 18610-034 Botucatu, Brazil., Figueiró HC; Green Biotech Network, School of Agricultural Sciences, São Paulo State University (UNESP), 18610-034 Botucatu, Brazil., Cicek SS; Department of Pharmaceutical Biology, Kiel University, Gutenbergstrase 76, 24118 Kiel, Germany.; Department of Biotechnology, Hamburg University of Applied Sciences, Ulmentliet 20, 21033 Hamburg, Germany., Spiegler V; Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, 48149 Münster, Germany., Funari CS; Green Biotech Network, School of Agricultural Sciences, São Paulo State University (UNESP), 18610-034 Botucatu, Brazil. |
| Abstrakt: |
The fact that alkaloids are bases has been the most explored chemical feature of their extraction and purification procedures. The main drawback of these procedures is that they employ undesirable chemicals, with HCl and CH 2 Cl 2 probably being the most commonly employed chemicals in their subsequent steps. This work tested the hypothesis that advantages in recovery efficiency support this common practice. Experiments were conducted in three laboratories, monitoring the alkaloids harmine ( 1 ), boldine ( 2 ), vincamine ( 3 ), and mescaline ( 4 ) extracted from Banisteriopsis caapi , Peumus boldus , Vinca minor , and Trichocereus macrogonus var. pachanoi , respectively. The research demonstrated that HCl could be replaced with citric acid (CA) without loss or even better extraction performance. The recommended EtOAc could completely replace CH 2 Cl 2 in three out of four study cases and partially in the fourth case without harming the extraction efficiency. In addition, the alternative solvents tert -amyl methyl ether (TAME) and n -butyl acetate (BuOAc) could enhance the extraction of alkaloids. These results might incentivize natural products laboratories to consider sustainability more routinely, thus being closer to current practices in the pharmaceutical industry, which has been replacing solvents and processes with greener ones. |
