A handheld luminometer with sub-attomole limit of detection for distributed applications in global health.

Autor:	Lebel P; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Elledge S; University of California, San Francisco, California, United States of America., Wiener DM; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Jeyakumar I; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Phelps M; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Jacobsen A; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Huynh E; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Charlton C; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Puccinelli R; University of California, Berkeley, California, United States of America., Mondal P; Child Health Research Foundation, Dhaka, Bangladesh., Saha S; Child Health Research Foundation, Dhaka, Bangladesh., Tato CM; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America., Gómez-Sjöberg R; Chan Zuckerberg Biohub San Francisco, San Francisco, California, United States of America.
Jazyk:	angličtina
Zdroj:	PLOS global public health [PLOS Glob Public Health] 2024 Feb 21; Vol. 4 (2), pp. e0002766. Date of Electronic Publication: 2024 Feb 21 (Print Publication: 2024).
DOI:	10.1371/journal.pgph.0002766
Abstrakt:	Luminescence is ubiquitous in biology research and medicine. Conceptually simple, the detection of luminescence nonetheless faces technical challenges because relevant signals can exhibit exceptionally low radiant power densities. Although low light detection is well-established in centralized laboratory settings, the cost, size, and environmental requirements of high-performance benchtop luminometers are not compatible with geographically-distributed global health studies or resource-constrained settings. Here we present the design and application of a ~$700 US handheld, battery-powered luminometer with performance on par with high-end benchtop instruments. By pairing robust and inexpensive Silicon Photomultiplier (SiPM) sensors with a low-profile shutter system, our design compensates for sensor non-idealities and thermal drift, achieving a limit of detection of 1.6E-19 moles of firefly luciferase. Using these devices, we performed two pilot cross-sectional serology studies to assess sars-cov-2 antibody levels: a cohort in the United States, as well as a field study in Bangladesh. Results from both studies were consistent with previous work and demonstrate the device's suitability for distributed applications in global health. Competing Interests: S.E. declares a previously filed provisional patent application on the solution-based spLUC assay. (Copyright: © 2024 Lebel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze:	MEDLINE
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