Engineering Synthetic circRNAs for Efficient CNS Expression.

Autor:	Clements KN; Department of Surgery, Duke University School of Medicine, Durham, NC, USA., Gonzalez TJ; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA., Asokan A; Department of Surgery, Duke University School of Medicine, Durham, NC, USA. aravind.asokan@duke.edu.; Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA. aravind.asokan@duke.edu.; Department of Biomedical Engineering, Duke University, Durham, NC, USA. aravind.asokan@duke.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2765, pp. 227-246.
DOI:	10.1007/978-1-0716-3678-7_13
Abstrakt:	Circular RNAs (circRNAs) have recently emerged as a promising modality for gene and RNA-based therapies. They are more stable than their linear counterpart and can be designed for efficient expression in different cell and tissue types. In this chapter, we developed different backsplicing circRNA cassettes that can enable efficient gene expression in various cell and tissue types. Furthermore, we packaged cassettes encoding circRNAs into adeno-associated viral (AAV) vectors that can be delivered via intracerebroventricular (ICV) injections to achieve expression in murine brain tissue. We provide detailed methods for the design of backsplicing circRNAs, circRNA detection, and generation of AAV-circRNA vectors for CNS dosing and expression in mice. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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