Probing efficient microbial CO 2 utilisation through metabolic and process modelling.

Autor: Gorter de Vries PJ; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Mol V; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark., Sonnenschein N; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark., Jensen TØ; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.; Again, Søborg, Denmark., Nielsen AT; The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2024 Feb; Vol. 17 (2), pp. e14414.
DOI: 10.1111/1751-7915.14414
Abstrakt: Acetogenic gas fermentation is increasingly studied as a promising technology to upcycle carbon-rich waste gasses. Currently the product range is limited, and production yields, rates and titres for a number of interesting products do not allow for economically viable processes. By pairing process modelling and host-agnostic metabolic modelling, we compare fermentation conditions and various products to optimise the processes. The models were then used in a simulation of an industrial-scale bubble column reactor. We find that increased temperatures favour gas transfer rates, particularly for the valuable and limiting H 2 , while furthermore predicting an optimal feed composition of 9:1 mol H 2 to mol CO 2 . Metabolically, the increased non-growth associated maintenance requirements of thermophiles favours the formation of catabolic products. To assess the expansion of the product portfolio beyond acetate, both a product volatility analysis and a metabolic pathway model were implemented. In-situ recovery of volatile products is shown to be within range for acetone but challenging due to the extensive evaporation of water, while the direct production of more valuable compounds by acetogens is metabolically unfavourable compared to acetate and ethanol. We discuss alternative approaches to overcome these challenges to utilise acetogenic CO 2 fixation to produce a wider range of carbon negative chemicals.
(© 2024 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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