The protein phosphorylation landscape in photosystem I of the desert algae Chlorella sp.

Autor: Levin G; Faculty of Biology, Technion, Haifa, 32000, Israel., Yasmin M; Faculty of Biology, Technion, Haifa, 32000, Israel., Pieńko T; Schulich Faculty of Chemistry, Technion, Haifa, 32000, Israel., Yehishalom N; Faculty of Biology, Technion, Haifa, 32000, Israel., Hanna R; Faculty of Biology, Technion, Haifa, 32000, Israel., Kleifeld O; Faculty of Biology, Technion, Haifa, 32000, Israel., Glaser F; The Lorry I. Lokey Center for Life Sciences and Engineering, Technion, Haifa, 32000, Israel., Schuster G; Faculty of Biology, Technion, Haifa, 32000, Israel.; Grand Technion Energy Program, Technion, Haifa, 32000, Israel.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Apr; Vol. 242 (2), pp. 544-557. Date of Electronic Publication: 2024 Feb 21.
DOI: 10.1111/nph.19603
Abstrakt: The phosphorylation of photosystem II (PSII) and its antenna (LHCII) proteins has been studied, and its involvement in state transitions and PSII repair is known. Yet, little is known about the phosphorylation of photosystem I (PSI) and its antenna (LHCI) proteins. Here, we applied proteomics analysis to generate a map of the phosphorylation sites of the PSI-LHCI proteins in Chlorella ohadii cells that were grown under low or extreme high-light intensities (LL and HL). Furthermore, we analyzed the content of oxidized tryptophans and PSI-LHCI protein degradation products in these cells, to estimate the light-induced damage to PSI-LHCI. Our work revealed the phosphorylation of 17 of 22 PSI-LHCI subunits. The analyses detected the extensive phosphorylation of the LHCI subunits Lhca6 and Lhca7, which is modulated by growth light intensity. Other PSI-LHCI subunits were phosphorylated to a lesser extent, including PsaE, where molecular dynamic simulation proposed that a phosphoserine stabilizes ferredoxin binding. Additionally, we show that HL-grown cells accumulate less oxidative damage and degradation products of PSI-LHCI proteins, compared with LL-grown cells. The significant phosphorylation of Lhca6 and Lhca7 at the interface with other LHCI subunits suggests a physiological role during photosynthesis, possibly by altering light-harvesting characteristics and binding of other subunits.
(© 2024 The Authors New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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