Modular Nanotransporters Capable of Causing Intracellular Degradation of the N-Protein of the SARS-CoV-2 Virus in A549 Cells with Temporary Expression of This Protein Fused with a Fluorescent Protein mRuby3.

Autor: Khramtsov YV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Ulasov AV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Lupanova TN; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Georgiev GP; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia., Sobolev AS; Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. alsobolev@yandex.ru.; Moscow State University, Moscow, Russia. alsobolev@yandex.ru.
Jazyk: angličtina
Zdroj: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2023 Dec; Vol. 513 (Suppl 1), pp. S60-S62. Date of Electronic Publication: 2024 Feb 20.
DOI: 10.1134/S1607672923700710
Abstrakt: Modular nanotransporters (MNTs) containing an antibody-like molecule, monobody, to the N‑protein of the SARS-CoV-2 virus, as well as an amino acid sequence that recruits the Keap1 E3 ligase (E3BP) were created. This MNT also included a site for cleavage of the E3BP monobody from the MNT in acidic endocytic compartments. It was shown that this cleavage by the endosomal protease cathepsin B leads to a 2.7-fold increase in the affinity of the E3BP monobody for the N-protein. Using A549 cells with transient expression of the N-protein fused with the fluorescent protein mRuby3, it was shown that incubation with MNT leads to a significant decrease in mRuby3 fluorescence. It is assumed that the developed MNTs can serve as a basis for the creation of new antiviral drugs against the SARS-CoV-2 virus.
(© 2023. Pleiades Publishing, Ltd.)
Databáze: MEDLINE
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