Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida.

Autor: Li H; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Akella S; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Engstler C; Department Biologie I-Botanik, Ludwig-Maximilians-Universität München, D-82152, Planegg-Martinsried, Germany., Omini JJ; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Rodriguez M; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Obata T; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.; Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Carrie C; School of Biological Sciences, University of Auckland, Auckland, 1142, New Zealand., Cerutti H; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.; School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA., Mower JP; Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA. jpmower@unl.edu.; Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. jpmower@unl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Feb 20; Vol. 15 (1), pp. 1548. Date of Electronic Publication: 2024 Feb 20.
DOI: 10.1038/s41467-024-45813-y
Abstrakt: Mitochondrial cytochrome c maturation (CCM) requires heme attachment via distinct pathways termed systems I and III. The mosaic distribution of these systems in Archaeplastida raises questions about the genetic mechanisms and evolutionary forces promoting repeated evolution. Here, we show a recurrent shift from ancestral system I to the eukaryotic-specific holocytochrome c synthase (HCCS) of system III in 11 archaeplastid lineages. Archaeplastid HCCS is sufficient to rescue mutants of yeast system III and Arabidopsis system I. Algal HCCS mutants exhibit impaired growth and respiration, and altered biochemical and metabolic profiles, likely resulting from deficient CCM and reduced cytochrome c-dependent respiratory activity. Our findings demonstrate that archaeplastid HCCS homologs function as system III components in the absence of system I. These results elucidate the evolutionary trajectory and functional divergence of CCM pathways in Archaeplastida, providing insight into the causes, mechanisms, and consequences of repeated cooption of an entire biological pathway.
(© 2024. The Author(s).)
Databáze: MEDLINE
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