Phosphorylation of plasma membrane H + -ATPase Thr881 participates in light-induced stomatal opening.

Autor: Hayashi Y; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Fukatsu K; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Takahashi K; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan.; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya, Japan., Kinoshita SN; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Kato K; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Sakakibara T; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan., Kuwata K; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya, Japan., Kinoshita T; Graduate School of Science, Nagoya University, Chikusa, Nagoya, Japan. kinoshita@bio.nagoya-u.ac.jp.; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Chikusa, Nagoya, Japan. kinoshita@bio.nagoya-u.ac.jp.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Feb 20; Vol. 15 (1), pp. 1194. Date of Electronic Publication: 2024 Feb 20.
DOI: 10.1038/s41467-024-45248-5
Abstrakt: Plasma membrane (PM) H + -ATPase is crucial for light-induced stomatal opening and phosphorylation of a penultimate residue, Thr948 (pen-Thr, numbering according to Arabidopsis AHA1) is required for enzyme activation. In this study, a comprehensive phosphoproteomic analysis using guard cell protoplasts from Vicia faba shows that both red and blue light increase the phosphorylation of Thr881, of PM H + -ATPase. Light-induced stomatal opening and the blue light-induced increase in stomatal conductance are reduced in transgenic Arabidopsis plants expressing mutant AHA1-T881A in aha1-9, whereas the blue light-induced phosphorylation of pen-Thr is unaffected. Auxin and photosynthetically active radiation induce the phosphorylation of both Thr881 and pen-Thr in etiolated seedlings and leaves, respectively. The dephosphorylation of phosphorylated Thr881 and pen-Thr are mediated by type 2 C protein phosphatase clade D isoforms. Taken together, Thr881 phosphorylation, in addition of the pen-Thr phosphorylation, are important for PM H + -ATPase function during physiological responses, such as light-induced stomatal opening in Arabidopsis thaliana.
(© 2024. The Author(s).)
Databáze: MEDLINE
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