Osteocalcin improves glucose tolerance, insulin sensitivity and secretion in older male mice.
Autor: | Paracha N; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Mastrokostas P; Department of Chemistry, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Kello E; Department of Chemistry, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Gedailovich Y; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Segall D; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Rizzo A; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Mitelberg L; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Hassan N; Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America., Dowd TL; Department of Chemistry, Brooklyn College of the City University of New York, Brooklyn, NY 11210, United States of America; Ph.D. Program in Chemistry and Ph.D. Program in Biochemistry, The Graduate Center of the City University of New York, New York, NY 10016, United States of America. Electronic address: TDowd@brooklyn.cuny.edu. |
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Jazyk: | angličtina |
Zdroj: | Bone [Bone] 2024 May; Vol. 182, pp. 117048. Date of Electronic Publication: 2024 Feb 18. |
DOI: | 10.1016/j.bone.2024.117048 |
Abstrakt: | Osteocalcin deficient mice (OC-/-), on a mixed 129/BL6J background, were reported to show glucose intolerance, insulin insensitivity and reduced insulin secretion at 1-6 mos of age. This is controversial as two studies in OC-/- mice on different backgrounds (C3H/BL6 (5-6 mos.) and C57BL/6N (5 and 9 mos.)) found no effect on glucose metabolism. To determine the role of OC in glucose metabolism we conducted glucose tolerance tests (GTT), insulin tolerances tests (ITT) and glucose stimulated insulin secretion (GSIS) on 6 and 9.5 month-old male OC-/- and OC+/+ mice on a pure C57BL/6J background and fed a normal chow diet. All results were analyzed with a two-way repeated measures ANOVA. The GTT results showed no effect on males at 6 months of age but glucose intolerance was significantly increased (p < 0.05) in male OC-/- mice at 9.5 months of age. The ITT results indicated significantly increased insulin resistance in male OC-/- mice. Glucose stimulated insulin secretion (GSIS) showed insulin significantly (p < 0.05) reduced in OC-/- at several time points. Mouse Osteocalcin injected into OC-/- mice decreased the glucose level. Our results confirm the role of OC in glucose metabolism and insulin sensitivity and demonstrate a role in insulin secretion in older male mice on a C57BL/6J background. Differences in background, age, or experimental procedures could explain controversial results. A delayed onset of the effect of OC on glucose metabolism at 9.5 months in male C57BL/6J mice highlights the importance of background on phenotype. Consideration of genetic background and age may be beneficial for human studies on osteocalcin and glucose homeostasis and may be relevant to the elderly where osteocalcin is reduced. Competing Interests: Declaration of competing interest No author on this manuscript has any Conflict of Interest to report. (Copyright © 2024 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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