Protocol to synthesize the auxin analog 5-Ph-IAA for conditional protein depletion in C. elegans using the AID2 system.

Autor:	Sural S; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY 10027, USA., Botero JQ; Department of Chemistry, Columbia University, New York, NY 10027, USA., Hobert O; Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY 10027, USA. Electronic address: or38@columbia.edu., Tekle-Smith M; Department of Chemistry, Columbia University, New York, NY 10027, USA. Electronic address: mt2992@columbia.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Mar 15; Vol. 5 (1), pp. 102901. Date of Electronic Publication: 2024 Feb 19.
DOI:	10.1016/j.xpro.2024.102901
Abstrakt:	The auxin-inducible degron (AID) system is a broadly used tool for spatiotemporal and reversible control of protein depletion in multiple experimental model systems. AID2 technology relies on a synthetic ligand, 5-phenyl-indole-3-acetic acid (5-Ph-IAA), for improved specificity and efficiency of protein degradation. Here, we provide a protocol for cost-effective 5-Ph-IAA synthesis utilizing the Suzuki coupling of 5-chloroindole and phenylboronic acid. We describe steps for evaluating the quality of lab-synthesized 5-Ph-IAA using a C. elegans AID2 tester strain. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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