Rice NLR protein XinN1, induced by a pattern recognition receptor XA21, confers enhanced resistance to bacterial blight.

Autor: Moon H; Department of Molecular Biology, Sejong University, Seoul, Republic of Korea.; Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA., Jeong AR; Department of Molecular Biology, Sejong University, Seoul, Republic of Korea., Park CJ; Department of Molecular Biology, Sejong University, Seoul, Republic of Korea. cjpark@sejong.ac.kr.; Department of Bioresources Engineering, Sejong University, Seoul, Republic of Korea. cjpark@sejong.ac.kr.
Jazyk: angličtina
Zdroj: Plant cell reports [Plant Cell Rep] 2024 Feb 20; Vol. 43 (3), pp. 72. Date of Electronic Publication: 2024 Feb 20.
DOI: 10.1007/s00299-024-03156-4
Abstrakt: Key Message: Rice CC-type NLR XinN1, specifically induced by a PRR XA21, activates defense pathways against Xoo. Plants have evolved two layers of immune systems regulated by two different types of immune receptors, cell surface located pattern recognition receptors (PRRs) and intracellular nucleotide-binding domain leucine-rich repeat-containing receptors (NLRs). Plant PRRs recognize conserved molecular patterns from diverse pathogens, resulting in pattern-triggered immunity (PTI), whereas NLRs are activated by effectors secreted by pathogens into plant cells, inducing effector-triggered immunity (ETI). Rice PRR, XA21, recognizes a tyrosine-sulfated RaxX peptide (required for activation of XA21-mediated immunity X) as a molecular pattern secreted by Xanthomonas oryzae pv. oryzae (Xoo). Here, we identified a rice NLR gene, XinN1, that is specifically induced during the XA21-mediated immune response against Xoo. Transgenic rice plants overexpressing XinN1 displayed increased resistance to infection by Xoo with reduced lesion length and bacterial growth. Overexpression of autoactive mutant of XinN1 (XinN1 D543V ) also displayed increased resistance to Xoo, accompanied with severe growth retardation and cell death. In rice protoplast system, overexpression of XinN1 or XinN1 D543V significantly elevated reactive oxygen species (ROS) production and cytosolic-free calcium (Ca 2+ ) accumulations. In addition, XinN1 overexpression additionally elevated the ROS burst caused by the interaction between XA21 and RaxX-sY and induced the transcription of PTI signaling components, including somatic embryogenesis receptor kinases (OsSERKs) and receptor-like cytoplasmic kinases (OsRLCKs). Our results suggest that XinN1 induced by the PRR XA21 activates defense pathways and provides enhanced resistance to Xoo in rice.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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