Shigella sonnei utilises colicins during inter-bacterial competition.

Autor:	Leung PB; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Matanza XM; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Roche B; Universite de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, CNRS UPR9002, F-67000 Strasbourg, France., Ha KP; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Cheung HC; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Appleyard S; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Collins T; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Flanagan O; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK., Marteyn BS; Universite de Strasbourg, Institut de Biologie Moléculaire et Cellulaire, CNRS UPR9002, F-67000 Strasbourg, France.; University of Strasbourg Institute for Advanced Study (USIAS), F-67000 Strasbourg, France.; Institut Pasteur, Université de Paris, Inserm U1225, Unité de Pathogenèse des Infections Vasculaires, F-75015 Paris, France., Clements A; Department of Life Sciences, South Kensington Campus, Imperial College London, London, SW72AZ, UK.
Jazyk:	angličtina
Zdroj:	Microbiology (Reading, England) [Microbiology (Reading)] 2024 Feb; Vol. 170 (2).
DOI:	10.1099/mic.0.001434
Abstrakt:	The mammalian colon is one of the most densely populated habitats currently recognised, with 10 11 -10 13 commensal bacteria per gram of colonic contents. Enteric pathogens must compete with the resident intestinal microbiota to cause infection. Among these enteric pathogens are Shigella species which cause approximately 125 million infections annually, of which over 90 % are caused by Shigella flexneri and Shigella sonnei. Shigella sonnei was previously reported to use a Type VI Secretion System (T6SS) to outcompete E. coli and S. flexneri in in vitro and in vivo experiments. S. sonnei strains have also been reported to harbour colicinogenic plasmids, which are an alternative anti-bacterial mechanism that could provide a competitive advantage against the intestinal microbiota. We sought to determine the contribution of both T6SS and colicins to the anti-bacterial killing activity of S. sonnei . We reveal that whilst the T6SS operon is present in S. sonnei, there is evidence of functional degradation of the system through SNPs, indels and IS within key components of the system. We created strains with synthetically inducible T6SS operons but were still unable to demonstrate anti-bacterial activity of the T6SS. We demonstrate that the anti-bacterial activity observed in our in vitro assays was due to colicin activity. We show that S. sonnei no longer displayed anti-bacterial activity against bacteria that were resistant to colicins, and removal of the colicin plasmid from S. sonnei abrogated anti-bacterial activity of S. sonnei . We propose that the anti-bacterial activity demonstrated by colicins may be sufficient for niche competition by S. sonnei within the gastrointestinal environment.
Databáze:	MEDLINE
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