Dietary resistant starch supplementation increases gut luminal deoxycholic acid abundance in mice.

Autor: Reuter MA; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Tucker M; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Marfori Z; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA., Shishani R; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Bustamante JM; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Moreno R; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Goodson ML; Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California - Davis, Davis, CA, USA., Ehrlich A; Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California - Davis, Davis, CA, USA., Taha AY; Department of Food Science and Technology, University of California - Davis, Davis, CA, USA., Lein PJ; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA., Joshi N; Bioinformatics Core, UC Davis Genome Center, University of California - Davis, Davis, CA, USA., Brito I; Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA., Durbin-Johnson B; Bioinformatics Core, UC Davis Genome Center, University of California - Davis, Davis, CA, USA., Nandakumar R; Biomarkers Core Laboratory, Irving Institute for Clinical and Translational Research, Columbia University Irving Medical Center, New York, NY, USA., Cummings BP; Department of Surgery, Center for Alimentary and Metabolic Sciences, School of Medicine, University of California - Davis, Sacramento, CA, USA.; Department of Molecular Biosciences, School of Veterinary Medicine, University of California - Davis, Davis, CA, USA.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2024 Jan-Dec; Vol. 16 (1), pp. 2315632. Date of Electronic Publication: 2024 Feb 20.
DOI: 10.1080/19490976.2024.2315632
Abstrakt: Bile acids (BA) are among the most abundant metabolites produced by the gut microbiome. Primary BAs produced in the liver are converted by gut bacterial 7-α-dehydroxylation into secondary BAs, which can differentially regulate host health via signaling based on their varying affinity for BA receptors. Despite the importance of secondary BAs in host health, the regulation of 7-α-dehydroxylation and the role of diet in modulating this process is incompletely defined. Understanding this process could lead to dietary guidelines that beneficially shift BA metabolism. Dietary fiber regulates gut microbial composition and metabolite production. We tested the hypothesis that feeding mice a diet rich in a fermentable dietary fiber, resistant starch (RS), would alter gut bacterial BA metabolism. Male and female wild-type mice were fed a diet supplemented with RS or an isocaloric control diet (IC). Metabolic parameters were similar between groups. RS supplementation increased gut luminal deoxycholic acid (DCA) abundance. However, gut luminal cholic acid (CA) abundance, the substrate for 7-α-dehydroxylation in DCA production, was unaltered by RS. Further, RS supplementation did not change the mRNA expression of hepatic BA producing enzymes or ileal BA transporters. Metagenomic assessment of gut bacterial composition revealed no change in the relative abundance of bacteria known to perform 7-α-dehydroxylation. P. ginsenosidimutans and P. multiformis were positively correlated with gut luminal DCA abundance and increased in response to RS supplementation. These data demonstrate that RS supplementation enriches gut luminal DCA abundance without increasing the relative abundance of bacteria known to perform 7-α-dehydroxylation.
Databáze: MEDLINE