| Autor: |
Witte TE; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario K1A 0C6, Canada.; Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada., Hicks C; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario K1A 0C6, Canada., Hermans A; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario K1A 0C6, Canada., Shields S; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario K1A 0C6, Canada., Overy DP; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario K1A 0C6, Canada. |
| Abstrakt: |
Fusarium poae is commonly detected in field surveys of Fusarium head blight (FHB) of cereal crops and can produce a range of trichothecene mycotoxins. Although experimentally validated reports of F. poae strains producing T-2/HT-2 trichothecenes are rare, F. poae is frequently generalized in the literature as a producer of T-2/HT-2 toxins due to a single study from 2004 in which T-2/HT-2 toxins were detected at low levels from six out of forty-nine F. poae strains examined. To validate/substantiate the observations reported from the 2004 study, the producing strains were acquired and phylogenetically confirmed to be correctly assigned as F. poae ; however, no evidence of T-2/HT-2 toxin production was observed from axenic cultures. Moreover, no evidence for a TRI16 ortholog, encoding a key acyltransferase shown to be necessary for T-2 toxin production in other Fusarium species, was observed in any of the de novo assembled genomes of the F. poae strains. Our findings corroborate multiple field-based and in vitro studies on FHB-associated Fusarium populations which also do not support the production of T-2/HT-2 toxins with F. poae and therefore conclude that F. poae should not be generalized as a T-2/HT-2 toxin producing species of Fusarium . |
