A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint.
| Autor: | Reddy VA; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore., Saju JM; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore., Nadimuthu K; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore., Sarojam R; Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in plant science [Front Plant Sci] 2024 Feb 05; Vol. 15, pp. 1284125. Date of Electronic Publication: 2024 Feb 05 (Print Publication: 2024). |
| DOI: | 10.3389/fpls.2024.1284125 |
| Abstrakt: | Phytohormone auxin controls various aspects of plant growth and development. The typical auxin signalling involves the degradation of canonical Aux/IAA proteins upon auxin perception releasing the auxin response factors (ARF) to activate auxin-regulated gene expression. Extensive research has been pursued in deciphering the role of canonical Aux/IAAs, however, the function of non-canonical Aux/IAA genes remains elusive. Here we identified a non-canonical Aux/IAA gene, MsIAA32 from spearmint ( Mentha spicata ), which lacks the TIR1-binding domain and shows its involvement in the development of peltate glandular trichomes (PGT), which are the sites for production and storage of commercially important essential oils. Using yeast two-hybrid studies, two canonical Aux/IAAs, MsIAA3, MsIAA4 and an ARF, MsARF3 were identified as the preferred binding partners of MsIAA32. Expression of a R2R3-MYB gene MsMYB36 and a cyclin gene MsCycB2-4 was altered in MsIAA32 suppressed plants indicating that these genes are possible downstream targets of MsIAA32 mediated signalling. Ectopic expression of MsIAA32 in Arabidopsis affected non-glandular trichome formation along with other auxin related developmental traits. Our findings establish the role of non-canonical Aux/IAA mediated auxin signalling in PGT development and reveal species-specific functionalization of Aux/IAAs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Reddy, Saju, Nadimuthu and Sarojam.) |
| Databáze: | MEDLINE |
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