Supervised and unsupervised machine learning approaches for monitoring subvisible particles within an aluminum-salt adjuvanted vaccine formulation.

Autor: Greenblott DN; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, USA., Wood CV; Merck & Co., Inc., Rahway, New Jersey, USA., Zhang J; Merck & Co., Inc., Rahway, New Jersey, USA., Viza N; Merck & Co., Inc., Rahway, New Jersey, USA., Chintala R; Merck & Co., Inc., Rahway, New Jersey, USA., Calderon CP; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, USA.; Ursa Analytics, Denver, Colorado, USA., Randolph TW; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, Colorado, USA.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2024 May; Vol. 121 (5), pp. 1626-1641. Date of Electronic Publication: 2024 Feb 19.
DOI: 10.1002/bit.28671
Abstrakt: Suspensions of protein antigens adsorbed to aluminum-salt adjuvants are used in many vaccines and require mixing during vial filling operations to prevent sedimentation. However, the mixing of vaccine formulations may generate undesirable particles that are difficult to detect against the background of suspended adjuvant particles. We simulated the mixing of a suspension containing a protein antigen adsorbed to an aluminum-salt adjuvant using a recirculating peristaltic pump and used flow imaging microscopy to record images of particles within the pumped suspensions. Supervised convolutional neural networks (CNNs) were used to analyze the images and create "fingerprints" of particle morphology distributions, allowing detection of new particles generated during pumping. These results were compared to those obtained from an unsupervised machine learning algorithm relying on variational autoencoders (VAEs) that were also used to detect new particles generated during pumping. Analyses of images conducted by applying both supervised CNNs and VAEs found that rates of generation of new particles were higher in aluminum-salt adjuvant suspensions containing protein antigen than placebo suspensions containing only adjuvant. Finally, front-face fluorescence measurements of the vaccine suspensions indicated changes in solvent exposure of tryptophan residues in the protein that occurred concomitantly with new particle generation during pumping.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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