In Vivo Cardiac Electrophysiology in Mice: Determination of Atrial and Ventricular Arrhythmic Substrates.

Autor: Navarro-Garcia JA; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas.; Department of Integrative Physiology, Baylor College of Medicine, Houston, Texas., Bruns F; Institute of Pharmacology, West German Heart and Vascular Center, University Duisburg-Essen, Essen, Germany., Moore OM; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas.; Department of Integrative Physiology, Baylor College of Medicine, Houston, Texas., Tekook MA; Institute of Pharmacology, West German Heart and Vascular Center, University Duisburg-Essen, Essen, Germany., Dobrev D; Department of Integrative Physiology, Baylor College of Medicine, Houston, Texas.; Institute of Pharmacology, West German Heart and Vascular Center, University Duisburg-Essen, Essen, Germany.; Department of Medicine and Research Center, Montreal Heart Institute and Université de Montréal, Montréal, Canada., Miyake CY; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas.; Department of Integrative Physiology, Baylor College of Medicine, Houston, Texas.; Department of Pediatrics, Division of Cardiology, Baylor College of Medicine, Houston, Texas., Wehrens XHT; Cardiovascular Research Institute, Baylor College of Medicine, Houston, Texas.; Department of Integrative Physiology, Baylor College of Medicine, Houston, Texas.; Department of Medicine, Division of Cardiology, Baylor College of Medicine, Houston, Texas.; Department of Neuroscience, Baylor College of Medicine, Houston, Texas.; Department of Pediatrics, Division of Cardiology, Baylor College of Medicine, Houston, Texas.; Center for Space Medicine, Baylor College of Medicine, Houston, Texas.
Jazyk: angličtina
Zdroj: Current protocols [Curr Protoc] 2024 Feb; Vol. 4 (2), pp. e994.
DOI: 10.1002/cpz1.994
Abstrakt: Cardiac arrhythmias are a common cardiac condition that might lead to fatal outcomes. A better understanding of the molecular and cellular basis of arrhythmia mechanisms is necessary for the development of better treatment modalities. To aid these efforts, various mouse models have been developed for studying cardiac arrhythmias. Both genetic and surgical mouse models are commonly used to assess the incidence and mechanisms of arrhythmias. Since spontaneous arrhythmias are uncommon in healthy young mice, intracardiac programmed electrical stimulation (PES) can be performed to assess the susceptibility to pacing-induced arrhythmias and uncover the possible presence of a proarrhythmogenic substrate. This procedure is performed by positioning an octopolar catheter inside the right atrium and ventricle of the heart through the right jugular vein. PES can provide insights into atrial and ventricular electrical activity and reveal whether atrial and/or ventricular arrhythmias are present or can be induced. Here, we explain detailed procedures used to perform this technique, possible troubleshooting scenarios, and methods to interpret the results obtained. © 2024 Wiley Periodicals LLC. Basic Protocol: Programmed electrical stimulation in mice.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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