Rapid typing of Klebsiella pneumoniae and Pseudomonas aeruginosa by Fourier-transform Infrared spectroscopy informs infection control in veterinary settings.
| Autor: | Zendri F; Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom., Schmidt V; Department of Small Animal Clinical Science, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom., Mauder N; Bruker Daltonics, Bremen, Germany., Loeffler A; Department of Clinical Science and Services, Royal Veterinary College Hawkshead Campus, Hatfield, Hertfordshire, United Kingdom., Jepson RE; Department of Clinical Science and Services, Royal Veterinary College Hawkshead Campus, Hatfield, Hertfordshire, United Kingdom., Isgren C; Western Counties Equine Hospital Ltd., Culmstock, United Kingdom., Pinchbeck G; Department of Livestock and One Health, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom., Haldenby S; Centre for Genomic Research, University of Liverpool, Liverpool, United Kingdom., Timofte D; Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2024 Feb 02; Vol. 15, pp. 1334268. Date of Electronic Publication: 2024 Feb 02 (Print Publication: 2024). |
| DOI: | 10.3389/fmicb.2024.1334268 |
| Abstrakt: | Introduction: The emergence of multi-drug resistant (MDR) pathogens linked to healthcare-associated infections (HCAIs) is an increasing concern in modern veterinary practice. Thus, rapid bacterial typing for real-time tracking of MDR hospital dissemination is still much needed to inform best infection control practices in a clinically relevant timeframe. To this end, the IR Biotyper using Fourier-Transform InfraRed (FTIR) spectroscopy has the potential to provide fast cluster analysis of potentially related organisms with substantial cost and turnaround time benefits. Materials and Methods: A collection of MDR bacterial isolates ( n = 199, comprising 92 Klebsiella pneumoniae and 107 Pseudomonas aeruginosa ) obtained from companion animal (i.e., dogs, cats and horses) clinical investigations, faecal and environmental screening from four veterinary facilities between 2012 and 2019 was analysed retrospectively by FTIR spectroscopy. Its performance was compared against MLST extracted from whole genomes of a subset of clustering isolates (proportionally to cluster size) for investigation of potential nosocomial transmission between patients and the surrounding hospital environments. Results: Concordance between the FTIR and MLST types was overall high for K. pneumoniae (Adjusted Rand Index [ARI] of 0.958) and poor for P. aeruginosa (ARI of 0.313). FTIR K. pneumoniae clusters ( n = 7) accurately segregated into their respective veterinary facility with evidence of intra-hospital spread of K. pneumoniae between patients and environmental surfaces. Notably, K. pneumoniae ST147 intensely circulated at one Small Animal Hospital ICU. Conversely, Pseudomonas aeruginosa FTIR clusters ( n = 18) commonly contained isolates of diversified hospital source and heterogeneous genetic background (as also genetically related isolates spread across different clusters); nonetheless, dissemination of some clones, such as P. aeruginosa ST2644 in the equine hospital, was apparent. Importantly, FTIR clustering of clinical, colonisation and/or environmental isolates sharing genomically similar backgrounds was seen for both MDR organisms, highlighting likely cross-contamination events that led to clonal dissemination within settings. Conclusion: FTIR spectroscopy has high discriminatory power for hospital epidemiological surveillance of veterinary K. pneumoniae and could provide sufficient information to support early detection of clonal dissemination, facilitating implementation of appropriate infection control measures. Further work and careful optimisation need to be carried out to improve its performance for typing of P. aeruginosa veterinary isolates. Competing Interests: NM is employed at Bruker Daltonics GmbH. CI was employed at the School of Veterinary Science, Philip Leverhulme Equine Hospital, University of Liverpool at the time of commencing the study and moved to Western Counties Equine Hospital Ltd where is currently employed. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Zendri, Schmidt, Mauder, Loeffler, Jepson, Isgren, Pinchbeck, Haldenby and Timofte.) |
| Databáze: | MEDLINE |
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