The interplay between gingival crevicular fluid microbiome and metabolomic profile in intensively treated people with type 1 diabetes - a combined metagenomic/metabolomic approach cross-sectional study.

Autor:	Gregorczyk-Maga I; Institute of Dentistry, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland., Kania M; Chair of Metabolic Diseases and Diabetology, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland.; Doctoral School of Medicine and Health Sciences, Jagiellonian University Medical College, Krakow, Poland., Dąbrowska M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Samborowska E; Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Żeber-Lubecka N; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.; Department of Gastroenterology, Hepatology and Clinical Oncology, Center of Postgraduate Medical Education, Warsaw, Poland., Kulecka M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.; Department of Gastroenterology, Hepatology and Clinical Oncology, Center of Postgraduate Medical Education, Warsaw, Poland., Klupa T; Center of Advanced Technologies in Diabetes, Chair of Metabolic Diseases, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland.
Jazyk:	angličtina
Zdroj:	Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2024 Feb 02; Vol. 14, pp. 1332406. Date of Electronic Publication: 2024 Feb 02 (Print Publication: 2023).
DOI:	10.3389/fendo.2023.1332406
Abstrakt:	Aims: This study aimed to assess the gingival crevicular fluid (GCF) microbiome and metabolome of adults with type 1 diabetes (T1D) treated with continuous subcutaneous insulin infusion (CSII). Methods: In this cross-sectional study, the GCF of adults with T1D treated with CSII and non-diabetic controls were sampled, and metagenomic/metabolomic analyses were performed. Results: In total, 65 participants with T1D and 45 healthy controls with a mean age of 27.05 ± 5.95 years were investigated. There were 22 cases of mild gingivitis (G) in the T1D group. There were no differences considering the Shannon and Chao indices and β-diversity between people with T1D and G, with T1D without G, and healthy controls. Differential taxa were identified, which were mainly enriched in people with T1D and G. Acetic acid concentration was higher in people with T1D, regardless of the presence of G, than in healthy controls. Propionic acid was higher in people with T1D and G than in healthy controls. Isobutyric and isovaleric acid levels were higher in individuals with T1D and G than in the other two subgroups. The concentration of valeric acid was lower and that of caproic acid was higher in people with T1D (regardless of gingival status) than in healthy controls. Conclusions: The identification of early changes in periodontal tissues by targeting the microbiome and metabolome could potentially enable effective prevention and initial treatment of periodontal disease in people with T1D. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Gregorczyk-Maga, Kania, Dąbrowska, Samborowska, Żeber-Lubecka, Kulecka and Klupa.)
Databáze:	MEDLINE
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